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	-[http://partsregistry.org/Part:BBa_I763007 I763007] not found;		-[http://partsregistry.org/Part:BBa_I763007 I763007] not found;
-	-Plac-cI-LacY is correct.	+	-Plac-CI-LacY is correct.

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Revision as of 14:00, 20 September 2007

09/17/07

• Ligations for:

-I763026 + I763019;

-I763026 + I763004;

-I763025 + J04031.

• We transform ligations and strake them on plates.

09/18/07

• We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.

09/19/07

• Miniprep for:

-I763028;

-Ptet-LacI-T-PLac-GFP;

-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).

• Digestion for:

-I763028 with Eco/Spe;

-Ptet-LacI-T-PLac-GFP with Eco/Spe;

-Ptet-LacI-GFP with Eco/Spe;

-I763007 with Eco/Xba;

-Plac-cI-LacY with Eco/Spe1;

• Band extraction from gel for all digestion and then we observe:

-I763028, Ptet-LacI-T-PLac-GFP are died;

-Ptet-LacI-GFP is correct;

-I763007 not found;

-Plac-CI-LacY is correct.

09/20/07

09/21/07

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