http://2007.igem.org/wiki/index.php?title=Week_12&feed=atom&action=historyWeek 12 - Revision history2024-03-29T11:53:31ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Week_12&diff=42693&oldid=prevFrancesca.bugane2 at 15:52, 26 October 20072007-10-26T15:52:40Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Bologna | Back]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Bologna<ins class="diffchange diffchange-inline">#Diary </ins>| Back]]</div></td></tr>
</table>Francesca.bugane2http://2007.igem.org/wiki/index.php?title=Week_12&diff=23849&oldid=prevSilvia.tamarri2 at 10:23, 8 October 20072007-10-08T10:23:23Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::7. The bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::7. The bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::8. Very few bacteria with [http://partsregistry.org/Part:BBa_I7630231 I763031] plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::8. Very few bacteria with [http://partsregistry.org/Part:BBa_I7630231 I763031] plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with I763031 plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763031 </ins>I763031<ins class="diffchange diffchange-inline">] </ins>plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td></tr>
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</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=23848&oldid=prevSilvia.tamarri2 at 10:22, 8 October 20072007-10-08T10:22:56Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test if our newly-constructed devices work, that is if they beam fluorescence when inducted with IPTG. To do so we performed 3 tests in parallel in 3 different tubes, that we name here A, B and C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test if our newly-constructed devices work, that is if they beam fluorescence when inducted with IPTG. To do so we performed 3 tests in parallel in 3 different tubes, that we name here A, B and C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::1. In tube A e in tube B we add 5ml of LB medium and 2 different colonies of [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::1. In tube A e in tube B we add 5ml of LB medium and 2 different colonies of [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::2. In tube C we add 5ml of LB medium and a colony of I763031 plasmid. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::2. In tube C we add 5ml of LB medium and a colony of <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763031 </ins>I763031<ins class="diffchange diffchange-inline">] </ins>plasmid. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::3. After 2 hours we measure the OD value of tubes A, B, C and it is around 0.5. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::3. After 2 hours we measure the OD value of tubes A, B, C and it is around 0.5. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::4. For tubes A, B, C we divided the bacteria fluid in two different tubes A1, A2; B1, B2; C1, C2. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::4. For tubes A, B, C we divided the bacteria fluid in two different tubes A1, A2; B1, B2; C1, C2. </div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=23847&oldid=prevSilvia.tamarri2 at 10:22, 8 October 20072007-10-08T10:22:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::6. We examine a bacteria fluid drop of tubes A1, A2, B1, B2, C1, C2 with our fluorescence microscope. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::6. We examine a bacteria fluid drop of tubes A1, A2, B1, B2, C1, C2 with our fluorescence microscope. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::7. The bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::7. The bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::8. Very few bacteria with I763031 plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::8. Very few bacteria with <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I7630231 </ins>I763031<ins class="diffchange diffchange-inline">] </ins>plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with I763031 plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with I763031 plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=23836&oldid=prevSilvia.tamarri2 at 09:58, 8 October 20072007-10-08T09:58:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763027;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763027 </ins>I763027<ins class="diffchange diffchange-inline">]</ins>;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763035(Spe/Pst1), (Xba/Pst1).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763035 </ins>I763035<ins class="diffchange diffchange-inline">] </ins>(Spe/Pst1), (Xba/Pst1).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion for:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion for:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028] with Eco/Spe;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028] with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763027 with Eco/Spe;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763027 </ins>I763027<ins class="diffchange diffchange-inline">] </ins>with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763035 with Eco/Spe;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763035 </ins>I763035<ins class="diffchange diffchange-inline">] </ins>with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/Xba;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/Xba;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763036 with Eco/Spe1;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763036 </ins>I763036<ins class="diffchange diffchange-inline">] </ins>with Eco/Spe1;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion and then we observe:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion and then we observe:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028], I763027 are died;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028], <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763027 </ins>I763027<ins class="diffchange diffchange-inline">] </ins>are died;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763035 is correct;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763035 </ins>I763035<ins class="diffchange diffchange-inline">] </ins>is correct;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] not found;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] not found;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763036 is correct.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763036 </ins>I763036<ins class="diffchange diffchange-inline">] </ins>is correct.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=23835&oldid=prevSilvia.tamarri2 at 09:43, 8 October 20072007-10-08T09:43:29Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion and then we observe:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion and then we observe:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028], <del class="diffchange diffchange-inline">Ptet-LacI-T-PLac-GFP </del>are died;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028], <ins class="diffchange diffchange-inline">I763027 </ins>are died;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-I763035 is correct;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-I763035 is correct;</div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=23832&oldid=prevSilvia.tamarri2 at 09:36, 8 October 20072007-10-08T09:36:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Ptet-LacI-T-PLac-GFP</del>;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763027</ins>;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Ptet-LacI-GFP</del>(Spe/Pst1), (Xba/Pst1).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763035</ins>(Spe/Pst1), (Xba/Pst1).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion for:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion for:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028] with Eco/Spe;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028] with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Ptet-LacI-T-PLac-GFP </del>with Eco/Spe;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763027 </ins>with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Ptet-LacI-GFP </del>with Eco/Spe;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763035 </ins>with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/Xba;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/Xba;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Plac-cI-LacY </del>with Eco/Spe1;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763036 </ins>with Eco/Spe1;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion and then we observe:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion and then we observe:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028], Ptet-LacI-T-PLac-GFP are died;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763028 I763028], Ptet-LacI-T-PLac-GFP are died;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Ptet-LacI-GFP </del>is correct;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763035 </ins>is correct;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] not found;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763007 I763007] not found;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Plac-CI-LacY </del>is correct.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763036 </ins>is correct.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 48:</td>
<td colspan="2" class="diff-lineno">Line 48:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test if our newly-constructed devices work, that is if they beam fluorescence when inducted with IPTG. To do so we performed 3 tests in parallel in 3 different tubes, that we name here A, B and C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test if our newly-constructed devices work, that is if they beam fluorescence when inducted with IPTG. To do so we performed 3 tests in parallel in 3 different tubes, that we name here A, B and C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::1. In tube A e in tube B we add 5ml of LB medium and 2 different colonies of [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::1. In tube A e in tube B we add 5ml of LB medium and 2 different colonies of [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::2. In tube C we add 5ml of LB medium and a colony of <del class="diffchange diffchange-inline">PLac-cI-GFP </del>plasmid. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::2. In tube C we add 5ml of LB medium and a colony of <ins class="diffchange diffchange-inline">I763031 </ins>plasmid. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::3. After 2 hours we measure the OD value of tubes A, B, C and it is around 0.5. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::3. After 2 hours we measure the OD value of tubes A, B, C and it is around 0.5. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::4. For tubes A, B, C we divided the bacteria fluid in two different tubes A1, A2; B1, B2; C1, C2. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::4. For tubes A, B, C we divided the bacteria fluid in two different tubes A1, A2; B1, B2; C1, C2. </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 63:</td>
<td colspan="2" class="diff-lineno">Line 63:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::6. We examine a bacteria fluid drop of tubes A1, A2, B1, B2, C1, C2 with our fluorescence microscope. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::6. We examine a bacteria fluid drop of tubes A1, A2, B1, B2, C1, C2 with our fluorescence microscope. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::7. The bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::7. The bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::8. Very few bacteria with <del class="diffchange diffchange-inline">PLac-cI-GFP </del>plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::8. Very few bacteria with <ins class="diffchange diffchange-inline">I763031 </ins>plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with <del class="diffchange diffchange-inline">PLac-cI-GFP </del>plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with <ins class="diffchange diffchange-inline">I763031 </ins>plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=23033&oldid=prevSilvia.tamarri2 at 09:29, 5 October 20072007-10-05T09:29:41Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">Revision as of 09:29, 5 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''09/21/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''09/21/07'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Meeting: definition of fluorescence test protocol. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Bologna | Back]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Bologna | Back]]</div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=22859&oldid=prevSilvia.tamarri2 at 15:13, 4 October 20072007-10-04T15:13:51Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:13, 4 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 47:</td>
<td colspan="2" class="diff-lineno">Line 47:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test if our newly-constructed devices work, that is if they beam fluorescence when inducted with IPTG. To do so we performed 3 tests in parallel in 3 different tubes, that we name here A, B and C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test if our newly-constructed devices work, that is if they beam fluorescence when inducted with IPTG. To do so we performed 3 tests in parallel in 3 different tubes, that we name here A, B and C.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::1. In tube A e in tube B we add 5ml of LB medium and 2 different colonies of I763019 plasmid. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::1. In tube A e in tube B we add 5ml of LB medium and 2 different colonies of <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763019 </ins>I763019<ins class="diffchange diffchange-inline">] </ins>plasmid. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::2. In tube C we add 5ml of LB medium and a colony of PLac-cI-GFP plasmid. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::2. In tube C we add 5ml of LB medium and a colony of PLac-cI-GFP plasmid. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::3. After 2 hours we measure the OD value of tubes A, B, C and it is around 0.5. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::3. After 2 hours we measure the OD value of tubes A, B, C and it is around 0.5. </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 54:</td>
<td colspan="2" class="diff-lineno">Line 54:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The analyzed fluid without IPTG (tubes A1, B1, C1) includes: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The analyzed fluid without IPTG (tubes A1, B1, C1) includes: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ml of original tube fluid; </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ml of original tube fluid; </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::<del class="diffchange diffchange-inline">-</del>-2.5ml of LB medium; </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::-2.5ml of LB medium; </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ul of kanamicin. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ul of kanamicin. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The analyzed fluid with IPTG (tube A2, B2, C2) includes: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The analyzed fluid with IPTG (tube A2, B2, C2) includes: </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 60:</td>
<td colspan="2" class="diff-lineno">Line 60:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ml of LB medium; </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ml of LB medium; </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ul of kanamicin; </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::-2.5ul of kanamicin; </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-50ul of 100mM IPTG. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::</ins>-50ul of 100mM IPTG. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::6. We examine a bacteria fluid drop of tubes A1, A2, B1, B2, C1, C2 with our fluorescence microscope. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::6. We examine a bacteria fluid drop of tubes A1, A2, B1, B2, C1, C2 with our fluorescence microscope. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::7. The bacteria with <del class="diffchange diffchange-inline"> </del>I763019 <del class="diffchange diffchange-inline"> </del>plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::7. The bacteria with <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763019 </ins>I763019<ins class="diffchange diffchange-inline">] </ins>plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::8. Very few bacteria with PLac-cI-GFP plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::8. Very few bacteria with PLac-cI-GFP plasmid with (C2) and without (C1) IPTG beam fluorescence. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with I763019 plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with PLac-cI-GFP plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*In conclusion, we see that bacteria with <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763019 </ins>I763019<ins class="diffchange diffchange-inline">] </ins>plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with PLac-cI-GFP plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_12&diff=22855&oldid=prevSilvia.tamarri2 at 15:10, 4 October 20072007-10-04T15:10:40Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:10, 4 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''09/20/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''09/20/07'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Testing our devices'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Testing our devices''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We <del class="diffchange diffchange-inline">want </del>to test if our newly-constructed devices work, that is if they beam <del class="diffchange diffchange-inline">flourescence </del>when inducted with IPTG. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We <ins class="diffchange diffchange-inline">wanted </ins>to test if our newly-constructed devices work, that is if they beam <ins class="diffchange diffchange-inline">fluorescence </ins>when inducted with IPTG<ins class="diffchange diffchange-inline">. To do so we performed 3 tests in parallel in 3 different tubes, that we name here A, B and C.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::1. In tube A e in tube B we add 5ml of LB medium and 2 different colonies of I763019 plasmid. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::2. In tube C we add 5ml of LB medium and a colony of PLac-cI-GFP plasmid. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::3. After 2 hours we measure the OD value of tubes A, B, C and it is around 0.5. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::4. For tubes A, B, C we divided the bacteria fluid in two different tubes A1, A2; B1, B2; C1, C2. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::5. We add 1mM IPTG to the solution into tubes A2, B2, C2. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* The analyzed fluid without IPTG (tubes A1, B1, C1) includes: </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::-2.5ml of original tube fluid; </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::--2.5ml of LB medium; </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::-2.5ul of kanamicin. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*The analyzed fluid with IPTG (tube A2, B2, C2) includes: </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::-2.5ml of original tube fluid; </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::-2.5ml of LB medium; </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::-2.5ul of kanamicin; </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">-50ul of 100mM IPTG. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::6. We examine a bacteria fluid drop of tubes A1, A2, B1, B2, C1, C2 with our fluorescence microscope. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::7. The bacteria with I763019 plasmid doesn’t beam fluorescence with (A2, B2) nor without (A1, B1) IPTG; </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">::8. Very few bacteria with PLac-cI-GFP plasmid with (C2) and without (C1) IPTG beam fluorescence. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*In conclusion, we see that bacteria with I763019 plasmid (tubes A1, B1, A2, B2) don't seem to work at all, and bacteria with PLac-cI-GFP plasmid (tube C1, C2) don't seem to work properly either. We plan other experiments for next week</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*In 2 different tubes we add 5ml of LB medium and 2 different colonies of [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*In another tube we add 5ml of LB medium and a colony of PLac-cI-GFP plasmid. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*After 2 hours we measure the OD value and it is around 0.5. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*For each original tube we divide the bacteria fluid in two different tubes.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*We add 1mM IPTG to the solution into one tube.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The analyzed fluid without IPTG includes:</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-2.5ml of original tube fluid, </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-2.5ml of LB medium, </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-2.5ul of kanamicin.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The analyzed fluid with IPTG includes: </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-2.5ml of original tube fluid, </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-2.5ml of LB medium, </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-2.5ul of kanamicin,</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-50ul of 100mM IPTG.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*We examine a bacteria fluid drop from of each tube with our fluorescence microscope. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid don’t beam fluorescence with and without IPTG; </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Very few bacteria with PLac-cI-GFP plasmid with and without IPTG beam fluorescence.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">In conclusion, we see that bacteria with [http://partsregistry.org/Part:BBa_I763019 I763019] plasmid don't seem to work at all, and bacteria with PLac-cI-GFP plasmid don't seem to work properly.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Silvia.tamarri2