Week 13
From 2007.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
::::-thus we decide to grow the OD value to have higher output value. | ::::-thus we decide to grow the OD value to have higher output value. | ||
| - | ::2. pTetR-LacI-pLac-cI-LacY-GFP | + | ::2. ''pTetR-LacI-pLac-cI-LacY-GFP'' |
::::- we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min). | ::::- we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min). | ||
::::- thus we decide to use lactose. | ::::- thus we decide to use lactose. | ||
Revision as of 12:13, 4 October 2007
- 09/24/07
- 09/25/07
- 09/26/07
Fluorescence test according to fluorescence issue protocol of:
- 1. pLac-GFP:
- -bacteria use to have the PMT output maximum value;
- -normalization of OD value at 0.5;
- -output value without IPTG: [0.22, 0.27]V;
- -output value with IPTG:
- -after 10min: [0.18,0.28]V;
- -after 20min: [0.41,0.46]V;
- -after 30min: [0.42,0.70]V;
- -after 60min: [0.48,0.80]V;
- -thus we decide to grow the OD value to have higher output value.
- 1. pLac-GFP:
- 2. pTetR-LacI-pLac-cI-LacY-GFP
- - we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min).
- - thus we decide to use lactose.
- 2. pTetR-LacI-pLac-cI-LacY-GFP
- 09/27/07
- 09/28/07
