Week 13
From 2007.igem.org
(Difference between revisions)
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::'''09/26/07''' | ::'''09/26/07''' | ||
| + | '''Fluorescence test according to [[Bologna University/Fluorescence issue fluorescence issue protocol]]''' of: | ||
| + | |||
| + | 1. plac-gfp: | ||
| + | -bacteria use to have the PMT output maximum value; | ||
| + | -normalization of OD value at 0.5; | ||
| + | - output value without IPTG: [0.22, 0.27]V; | ||
| + | -output value with IPTG: | ||
| + | - after 10min: [0.18,0.28]V; | ||
| + | -after 20min: [0.41,0.46]V; | ||
| + | -after 30min: [0.42,0.70]V; | ||
| + | -after 60min: [0.48,0.80]V; | ||
| + | -thus we decide to grow the OD value to have higher output value. | ||
| + | |||
| + | 2. pTetR-LacI-pLac-cI-LacY-GFP | ||
| + | - we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min). | ||
| + | - thus we decide to use lactose. | ||
Revision as of 12:09, 4 October 2007
- 09/24/07
- 09/25/07
- 09/26/07
Fluorescence test according to Bologna University/Fluorescence issue fluorescence issue protocol of:
1. plac-gfp: -bacteria use to have the PMT output maximum value; -normalization of OD value at 0.5; - output value without IPTG: [0.22, 0.27]V; -output value with IPTG:
- after 10min: [0.18,0.28]V;
-after 20min: [0.41,0.46]V;
-after 30min: [0.42,0.70]V;
-after 60min: [0.48,0.80]V;
-thus we decide to grow the OD value to have higher output value.
2. pTetR-LacI-pLac-cI-LacY-GFP - we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min). - thus we decide to use lactose.
- 09/27/07
- 09/28/07
