Week 14

From 2007.igem.org

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In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
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Then, we have to set the
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Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
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Revision as of 13:49, 3 October 2007

10/01/07



10/02/07

Experimental Setup

To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a 10 seconds interval from the photomultiplier output.

In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.

Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.


10/03/07



10/04/07



10/05/07



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