Week 14

From 2007.igem.org

(Difference between revisions)
Line 16: Line 16:
::'''10/03/07'''
::'''10/03/07'''
-
*Digestion of:
+
*Digestions of:
::-[http://partsregistry.org/Part:I763032 I763032] with Eco/Pst1;
::-[http://partsregistry.org/Part:I763032 I763032] with Eco/Pst1;
::-[http://partsregistry.org/Part:I763031 I763031] with Eco/Pst1;
::-[http://partsregistry.org/Part:I763031 I763031] with Eco/Pst1;
Line 22: Line 22:
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
*Run on electrophoresis gel.
*Run on electrophoresis gel.
-
*[http://partsregistry.org/Part:I763031 I763031], +[http://partsregistry.org/Part:I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
+
*[http://partsregistry.org/Part:I763031 I763031], [http://partsregistry.org/Part:I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
-
*Transformation of  
+
*Transformations of:
::-[http://partsregistry.org/Part:I763034 I763034];  
::-[http://partsregistry.org/Part:I763034 I763034];  
::-[http://partsregistry.org/Part:I763031 I763031];
::-[http://partsregistry.org/Part:I763031 I763031];
::-[http://partsregistry.org/Part:I763032 I763032].
::-[http://partsregistry.org/Part:I763032 I763032].
-
*Ligation for
+
*Ligations for:
::-[http://partsregistry.org/Part:I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
::-[http://partsregistry.org/Part:I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
::-[http://partsregistry.org/Part:I763032 I763032::-[http://partsregistry.org/Part:I763032 I763032].
::-[http://partsregistry.org/Part:I763032 I763032::-[http://partsregistry.org/Part:I763032 I763032].

Revision as of 10:36, 8 October 2007

10/01/07
  • Meeting: Ethical issue.


10/02/07
  • Digestion of pSB4A3;
  • Run on electrophoresis gel.
  • pSB4A3; and I763033 band extraction.
  • Ligation for:
-I763004+I763033;
-I763012+I763020(I763032).



10/03/07
  • Digestions of:
-I763032 with Eco/Pst1;
-I763031 with Eco/Pst1;
-I763036 with Eco/Pst1;
-pSB4A3 with Eco/Pst1.
-I763034;
-I763031;
-I763032.
  • Ligations for:
-I763031+pSB4A3;
-I763032::-[http://partsregistry.org/Part:I763032 I763032.

Experimental Setup

  • To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
  • In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
  • Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.


10/04/07
  • Trasformation of
-I763031+pSB4A3;
-I763032+pSB4A3;



10/05/07
  • Stock preparation (500 ul) of I763034,I763031+pSB4A3 and I763032+pSB4A3.
  • Miniprep of I763034,I763031+pSB4A3 and I763032+pSB4A3.
  • Control digestion of
-I763034 with Xba/Pst1;
-I763031 with Eco/Xba and Xba/Pst1;
-I763032 with Eco/Xba and Xba/Pst1.
  • Run on electrophoresis gel and I763032 (Eco/Xba and Xba/Pst1) band extraction.


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