Week 4

From 2007.igem.org

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::'''07/23/07'''
::'''07/23/07'''
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*The uncorrected grew on the plate of I13507 and none of R0051.
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*Another transformation of R0051 (2 ul), I13507 and C0051.
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*Ligation of J04500*J04631 (I763004) to test the ligation protocoll with 2 experiences: vector and insert 2 ul, vector and insert 2-4 ul. We leave it at 25°C for an hour, then at 65°C for 10 minutes.
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* Transformation 

Revision as of 09:12, 27 August 2007

07/23/07
  • The uncorrected grew on the plate of I13507 and none of R0051.
  • Another transformation of R0051 (2 ul), I13507 and C0051.
  • Ligation of J04500*J04631 (I763004) to test the ligation protocoll with 2 experiences: vector and insert 2 ul, vector and insert 2-4 ul. We leave it at 25°C for an hour, then at 65°C for 10 minutes.
  • Transformation


07/24/07



07/25/07



07/26/07



07/27/07



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