Week 4
From 2007.igem.org
(Difference between revisions)
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::'''07/23/07''' | ::'''07/23/07''' | ||
| - | * | + | *We observed the uncorrected grew of I13507 on the plate and none of R0051. |
| - | * | + | *We got along with: |
| - | + | - another transformation of R0051 (2 ul), I13507 and C0051; | |
| - | * | + | |
| + | - the ligation of J04500+J04631 (I763004) to test the ligation protocoll with 2 experiences: vector and insert 2 ul, vector and insert 2-4 ul. We leave it at 25°C for an hour, then at 65°C for 10 minutes. | ||
| + | *Then we transformed 4 ul of ligate. | ||
Revision as of 09:21, 27 August 2007
- 07/23/07
- We observed the uncorrected grew of I13507 on the plate and none of R0051.
- We got along with:
- another transformation of R0051 (2 ul), I13507 and C0051;
- the ligation of J04500+J04631 (I763004) to test the ligation protocoll with 2 experiences: vector and insert 2 ul, vector and insert 2-4 ul. We leave it at 25°C for an hour, then at 65°C for 10 minutes.
- Then we transformed 4 ul of ligate.
- 07/24/07
- 07/25/07
- 07/26/07
- 07/27/07
