http://2007.igem.org/wiki/index.php?title=Week_4&feed=atom&action=historyWeek 4 - Revision history2024-03-28T23:53:48ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Week_4&diff=42678&oldid=prevSilvia.tamarri2 at 15:50, 26 October 20072007-10-26T15:50:58Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Bologna | Back]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Bologna<ins class="diffchange diffchange-inline">#Diary </ins>| Back]]</div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_4&diff=33435&oldid=prevSilvia.tamarri2 at 09:42, 24 October 20072007-10-24T09:42:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in 5ml in the evening and we leave them O/N.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in 5ml in the evening and we leave them O/N.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We perform a fluorescence test of [http://partsregistry.org/Part:BBa_J04431 J04431] and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the [[Bologna/Ligation protocol |ligation protocol]].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We perform a fluorescence test of [http://partsregistry.org/Part:BBa_J04431 J04431] and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the [[Bologna <ins class="diffchange diffchange-inline">University</ins>/Ligation protocol |ligation protocol]].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we inoculate in 5ml O/N.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we inoculate in 5ml O/N.</div></td></tr>
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</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_4&diff=33428&oldid=prevSilvia.tamarri2 at 09:41, 24 October 20072007-10-24T09:41:13Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in 5ml in the evening and we leave them O/N.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in 5ml in the evening and we leave them O/N.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We perform a fluorescence test of [http://partsregistry.org/Part:BBa_J04431 J04431] and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the ligation protocol.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We perform a fluorescence test of [http://partsregistry.org/Part:BBa_J04431 J04431] and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the <ins class="diffchange diffchange-inline">[[Bologna/Ligation protocol |</ins>ligation protocol<ins class="diffchange diffchange-inline">]]</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we inoculate in 5ml O/N.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we inoculate in 5ml O/N.</div></td></tr>
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</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_4&diff=33422&oldid=prevSilvia.tamarri2 at 09:39, 24 October 20072007-10-24T09:39:40Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in 5ml in the evening and we leave them O/N.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in 5ml in the evening and we leave them O/N.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We perform a fluorescence test of J04431 and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the ligation protocol.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We perform a fluorescence test of <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_J04431 </ins>J04431<ins class="diffchange diffchange-inline">] </ins>and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the ligation protocol.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we inoculate in 5ml O/N.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we inoculate in 5ml O/N.</div></td></tr>
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</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_4&diff=18267&oldid=prevSilvia.tamarri2 at 17:01, 9 September 20072007-09-09T17:01:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Transformation again for [http://partsregistry.org/Part:BBa_R0051 R0051] (2ul), [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_C0051 C0051];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Transformation again for [http://partsregistry.org/Part:BBa_R0051 R0051] (2ul), [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_C0051 C0051];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test ligation protocol. We try with vector and insert 2ul, vector and insert 2-4ul. We leave it at 25°C 1 h and at 65°C for 10 minutes. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test ligation protocol. We try with vector and insert 2ul, vector and insert 2-4ul. We leave it at 25°C <ins class="diffchange diffchange-inline">for </ins>1 h and at 65°C for 10 minutes. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we transform 4ul from the ligation reaction. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we transform 4ul from the ligation reaction. </div></td></tr>
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</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_4&diff=17925&oldid=prevFrancesca.bugane2 at 10:32, 5 September 20072007-09-05T10:32:09Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We measure OD every 20 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We measure OD every 20 minutes.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We <del class="diffchange diffchange-inline">get </del>along with another GFP induction: after 10 minutes the cells are already green.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We <ins class="diffchange diffchange-inline">go </ins>along with another GFP induction: after 10 minutes the cells are already green.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We transform bacteria with 4ul of the [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051] and with 10ul from [http://partsregistry.org/Part:BBa_J04500 J04500] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We transform bacteria with 4ul of the [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051] and with 10ul from [http://partsregistry.org/Part:BBa_J04500 J04500] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates.</div></td></tr>
</table>Francesca.bugane2http://2007.igem.org/wiki/index.php?title=Week_4&diff=17902&oldid=prevFrancesca.bugane2 at 09:26, 5 September 20072007-09-05T09:26:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/23/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/23/07'''</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*On the plates we observe the wrong growth of [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051]. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*On the plates we observe the wrong growth of [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051]. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Transformation again for [http://partsregistry.org/Part:BBa_R0051 R0051] (<del class="diffchange diffchange-inline">2 ul</del>), [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_C0051 C0051];</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Transformation again for [http://partsregistry.org/Part:BBa_R0051 R0051] (<ins class="diffchange diffchange-inline">2ul</ins>), [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_C0051 C0051];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test ligation protocol. We try with vector and insert <del class="diffchange diffchange-inline">2 ul</del>, vector and insert 2-<del class="diffchange diffchange-inline">4 ul</del>. We leave it at 25°C 1 h and at 65°C for 10 minutes. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test ligation protocol. We try with vector and insert <ins class="diffchange diffchange-inline">2ul</ins>, vector and insert 2-<ins class="diffchange diffchange-inline">4ul</ins>. We leave it at 25°C 1 h and at 65°C for 10 minutes. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Then we transform <del class="diffchange diffchange-inline">4 ul </del>from the ligation reaction. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Then we transform <ins class="diffchange diffchange-inline">4ul </ins>from the ligation reaction. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/24/07''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/24/07''' </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We observe colonies for [http://partsregistry.org/Part:BBa_C0051 C0051] plate. We inoculate in <del class="diffchange diffchange-inline">50 ml </del>and we leave at 37°C O/N.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We observe colonies for [http://partsregistry.org/Part:BBa_C0051 C0051] plate. We inoculate in <ins class="diffchange diffchange-inline">50ml </ins>and we leave at 37°C O/N.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We don’t observe any colony for [http://partsregistry.org/Part:BBa_R0051 R0051],[http://partsregistry.org/Part:BBa_I13507 I13507] and for the 2 ligations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We don’t observe any colony for [http://partsregistry.org/Part:BBa_R0051 R0051],[http://partsregistry.org/Part:BBa_I13507 I13507] and for the 2 ligations.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''GFP induction test''' in [http://partsregistry.org/Part:BBa_J04431 J04431] transformed cells: we inoculate in <del class="diffchange diffchange-inline">5 ml</del>. At OD: 0.4/0.6 + IPTG, we put a drop on the micro slide. After 30 minutes the cells are already green.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''GFP induction test''' in [http://partsregistry.org/Part:BBa_J04431 J04431] transformed cells: we inoculate in <ins class="diffchange diffchange-inline">5ml</ins>. At OD: 0.4/0.6 + IPTG, we put a drop on the micro slide. After 30 minutes the cells are already green.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We harvest and put them in <del class="diffchange diffchange-inline">5 ml </del>without IPTG to see the protein extinction time.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We harvest and put them in <ins class="diffchange diffchange-inline">5ml </ins>without IPTG to see the protein extinction time.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We measure OD every 20 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We measure OD every 20 minutes.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with another GFP induction: after 10 minutes the cells are already green.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with another GFP induction: after 10 minutes the cells are already green.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We transform bacteria with <del class="diffchange diffchange-inline">4 ul </del>of the [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051] and with <del class="diffchange diffchange-inline">10 ul </del>from [http://partsregistry.org/Part:BBa_J04500 J04500] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligations.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We transform bacteria with <ins class="diffchange diffchange-inline">4ul </ins>of the [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051] and with <ins class="diffchange diffchange-inline">10ul </ins>from [http://partsregistry.org/Part:BBa_J04500 J04500] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 23:</td>
<td colspan="2" class="diff-lineno">Line 24:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/25/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/25/07'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Midiprep for [http://partsregistry.org/Part:BBa_C0051 C0051]:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Midiprep for [http://partsregistry.org/Part:BBa_C0051 C0051]:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Midiprep Quantification: conc. <del class="diffchange diffchange-inline">65 ng</del>/ul;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Midiprep Quantification: conc. <ins class="diffchange diffchange-inline">65ng</ins>/ul;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We observe colonies for [http://partsregistry.org/Part:BBa_I13507 I13507] and ligation. We inoculate in <del class="diffchange diffchange-inline">50 ml</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We observe colonies for [http://partsregistry.org/Part:BBa_I13507 I13507] and ligation. We inoculate in <ins class="diffchange diffchange-inline">50ml</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We don’t find any colony for [http://partsregistry.org/Part:BBa_R0051 R0051]. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We don’t find any colony for [http://partsregistry.org/Part:BBa_R0051 R0051]. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We transform again with [http://partsregistry.org/Part:BBa_R0051 R0051] and with [http://partsregistry.org/Part:BBa_R1051 R1051].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We transform again with [http://partsregistry.org/Part:BBa_R0051 R0051] and with [http://partsregistry.org/Part:BBa_R1051 R1051].</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 33:</td>
<td colspan="2" class="diff-lineno">Line 34:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/26/07'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in <del class="diffchange diffchange-inline">5 ml </del>in the evening and we leave them O/N.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We let colonies from [http://partsregistry.org/Part:BBa_R0051 R0051] and from[http://partsregistry.org/Part:BBa_R1051 R1051] grow at 37°C during the day. We inoculate in <ins class="diffchange diffchange-inline">5ml </ins>in the evening and we leave them O/N.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We perform a fluorescence test of J04431 and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the ligation <del class="diffchange diffchange-inline">protocoll</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We perform a fluorescence test of J04431 and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) ligation to test the ligation <ins class="diffchange diffchange-inline">protocol</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Then we inoculate in <del class="diffchange diffchange-inline">5 ml </del>O/N.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Then we inoculate in <ins class="diffchange diffchange-inline">5ml </ins>O/N.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 41:</td>
<td colspan="2" class="diff-lineno">Line 42:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/27/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/27/07'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Miniprep for [http://partsregistry.org/Part:BBa_R0051 R0051] and [http://partsregistry.org/Part:BBa_R1051 R1051]. We don’t try to quantificate with <del class="diffchange diffchange-inline">spectofotometer </del>because it isn’t sensible enough. Midi for [http://partsregistry.org/Part:BBa_I13507 I13507]:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Miniprep for [http://partsregistry.org/Part:BBa_R0051 R0051] and [http://partsregistry.org/Part:BBa_R1051 R1051]. We don’t try to quantificate with <ins class="diffchange diffchange-inline">spectrophotometer </ins>because it isn’t sensible enough. Midi for [http://partsregistry.org/Part:BBa_I13507 I13507]:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Quantification: conc.:56 ng/ul.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Quantification: conc.:56 ng/ul.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Digestion for : </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Digestion for: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I13507 I13507] with XbaI/PstI;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I13507 I13507] with XbaI/PstI;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 50:</td>
<td colspan="2" class="diff-lineno">Line 51:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_C0051 C0051] with XbaI/PstI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_C0051 C0051] with XbaI/PstI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we extract from gel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we extract from gel.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We observe green fluorescence in [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) and [http://partsregistry.org/Part:BBa_J04431 J04431] cells not yet induced with IPTG. So, we decide to identify the moment in the bacterial growth when the fluorescence appears to understand if it is caused by glucose depletion in the culture medium and/or by <del class="diffchange diffchange-inline"> </del>a low LacI amount inside the cell. <del class="diffchange diffchange-inline">Infact </del>the endogenous LacI in E. Coli is sufficient for one Plac copy and we suspect it can’t <del class="diffchange diffchange-inline"> </del>repress Plac from the high copy number plasmid we have used. (We will perform this test on 31/07)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We observe green fluorescence in [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763004 I763004]) and [http://partsregistry.org/Part:BBa_J04431 J04431] cells not yet induced with IPTG. So, we decide to identify the moment in the bacterial growth when the fluorescence appears to understand if it is caused by glucose depletion in the culture medium and/or by a low LacI amount inside the cell. <ins class="diffchange diffchange-inline">In fact </ins>the endogenous LacI in E. Coli is sufficient for one Plac copy and we suspect it can’t repress Plac from the high copy number plasmid we have used. (We will perform this test on 31/07)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Francesca.bugane2http://2007.igem.org/wiki/index.php?title=Week_4&diff=17798&oldid=prevFrancesca.bugane2 at 13:23, 4 September 20072007-09-04T13:23:04Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:23, 4 September 2007</td>
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<td colspan="2" class="diff-lineno">Line 50:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_C0051 C0051] with XbaI/PstI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_C0051 C0051] with XbaI/PstI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we extract from gel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Then we extract from gel.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We observe green fluorescence in [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] (I763004) and [http://partsregistry.org/Part:BBa_J04431 J04431] cells not yet induced with IPTG. So, we decide to identify the moment in the bacterial growth when the fluorescence appears to understand if it is caused by glucose depletion in the culture medium and/or by a low LacI amount inside the cell. Infact the endogenous LacI in E. Coli is sufficient for one Plac copy and we suspect it can’t repress Plac from the high copy number plasmid we have used. (We will perform this test on 31/07)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We observe green fluorescence in [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_J04631 J04631] (<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763004 </ins>I763004<ins class="diffchange diffchange-inline">]</ins>) and [http://partsregistry.org/Part:BBa_J04431 J04431] cells not yet induced with IPTG. So, we decide to identify the moment in the bacterial growth when the fluorescence appears to understand if it is caused by glucose depletion in the culture medium and/or by a low LacI amount inside the cell. Infact the endogenous LacI in E. Coli is sufficient for one Plac copy and we suspect it can’t repress Plac from the high copy number plasmid we have used. (We will perform this test on 31/07)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Francesca.bugane2http://2007.igem.org/wiki/index.php?title=Week_4&diff=17797&oldid=prevFrancesca.bugane2 at 13:21, 4 September 20072007-09-04T13:21:38Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:21, 4 September 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/25/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/25/07'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Midiprep for [http://partsregistry.org/Part:BBa_C0051 C0051]:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Midiprep for [http://partsregistry.org/Part:BBa_C0051 C0051]:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">-</del>Midiprep Quantification: conc. 65 ng/ul;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*</ins>Midiprep Quantification: conc. 65 ng/ul;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We observe colonies for [http://partsregistry.org/Part:BBa_I13507 I13507] and ligation. We inoculate in 50 ml.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We observe colonies for [http://partsregistry.org/Part:BBa_I13507 I13507] and ligation. We inoculate in 50 ml.</div></td></tr>
</table>Francesca.bugane2http://2007.igem.org/wiki/index.php?title=Week_4&diff=17796&oldid=prevFrancesca.bugane2 at 13:18, 4 September 20072007-09-04T13:18:01Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:18, 4 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 12:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We don’t observe any colony for [http://partsregistry.org/Part:BBa_R0051 R0051],[http://partsregistry.org/Part:BBa_I13507 I13507] and for the 2 ligations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We don’t observe any colony for [http://partsregistry.org/Part:BBa_R0051 R0051],[http://partsregistry.org/Part:BBa_I13507 I13507] and for the 2 ligations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''GFP induction test''' in [http://partsregistry.org/Part:BBa_J04431 J04431] transformed cells: we inoculate in 5 ml. At OD: 0.4/0.6 + IPTG, we put a drop on the micro slide. After 30 minutes the cells are already green.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''GFP induction test''' in [http://partsregistry.org/Part:BBa_J04431 J04431] transformed cells: we inoculate in 5 ml. At OD: 0.4/0.6 + IPTG, we put a drop on the micro slide. After 30 minutes the cells are already green.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We harvest and put them in 5 ml without IPTG to see the extinction time <del class="diffchange diffchange-inline">of the protein</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We harvest and put them in 5 ml without IPTG to see the <ins class="diffchange diffchange-inline">protein </ins>extinction time.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We measure OD every 20 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We measure OD every 20 minutes.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with another GFP induction: after 10 minutes the cells are already green.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with another GFP induction: after 10 minutes the cells are already green.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We transform bacteria with 4 ul of the [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051] and with 10 ul from [http://partsregistry.org/Part:BBa_J04500 J04500] + [http://partsregistry.org/Part:BBa_J04631 J04631] (I763004) ligations.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We transform bacteria with 4 ul of the [http://partsregistry.org/Part:BBa_I13507 I13507] and [http://partsregistry.org/Part:BBa_R0051 R0051] and with 10 ul from [http://partsregistry.org/Part:BBa_J04500 J04500] + [http://partsregistry.org/Part:BBa_J04631 J04631] (<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763004 </ins>I763004<ins class="diffchange diffchange-inline">]</ins>) ligations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Francesca.bugane2