Week 5

From 2007.igem.org

(Difference between revisions)
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::'''07/31/07'''
::'''07/31/07'''
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*There isn’t any colony for C0012, so we inoculate I52034 and B0015 colony in 5 ml.
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*We plate 30/07 ligations.
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*We get along with the J04431 fluorescence test:
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-From the glycerol stock we sprout 1 ml at 37°C for 1 hour in agitation.
 +
-We verify the OD and the fluorescence every 15 minutes. The fluorescence compare at the istance t=210min and at the OD=0.5.
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*At the end of this test we decide to repeat it to see if we can switch off the fluorescent cells with a major quantity of glucose to confirm our assumptions.
 +
We will attend this test on 07/08.

Revision as of 12:17, 27 August 2007

07/30/07
  • We control bacterial grew (link).
  • Transformation for C0012 and J52034 and B0015.
  • We get along with the ligation R0051+I13507 (I763007) (vector 2 ul, insert 6 ul) and the ligation J04500+ C0051 (I763005) (vector 2 ul, insert 10 ul).



07/31/07
  • There isn’t any colony for C0012, so we inoculate I52034 and B0015 colony in 5 ml.
  • We plate 30/07 ligations.
  • We get along with the J04431 fluorescence test:

-From the glycerol stock we sprout 1 ml at 37°C for 1 hour in agitation. -We verify the OD and the fluorescence every 15 minutes. The fluorescence compare at the istance t=210min and at the OD=0.5.

  • At the end of this test we decide to repeat it to see if we can switch off the fluorescent cells with a major quantity of glucose to confirm our assumptions.

We will attend this test on 07/08.



08/01/07



08/02/07



08/03/07



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