http://2007.igem.org/wiki/index.php?title=Week_5&feed=atom&action=history
Week 5 - Revision history
2024-03-29T05:17:30Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2007.igem.org/wiki/index.php?title=Week_5&diff=42683&oldid=prev
Silvia.tamarri2 at 15:51, 26 October 2007
2007-10-26T15:51:18Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:51, 26 October 2007</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Bologna | Back]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Bologna<ins class="diffchange diffchange-inline">#Diary </ins>| Back]]</div></td></tr>
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Silvia.tamarri2
http://2007.igem.org/wiki/index.php?title=Week_5&diff=18671&oldid=prev
Guido Costa at 13:45, 12 September 2007
2007-09-12T13:45:07Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 13:45, 12 September 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- [http://partsregistry.org/Part:BBa_R0051 R0051] + [http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] fluorescence test to check if the RFP filter works properly.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- [http://partsregistry.org/Part:BBa_R0051 R0051] + [http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] fluorescence test to check if the RFP filter works properly.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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Guido Costa
http://2007.igem.org/wiki/index.php?title=Week_5&diff=18670&oldid=prev
Silvia.tamarri2 at 13:39, 12 September 2007
2007-09-12T13:39:34Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Every 15 minutes we check bacterial OD and fluorescence. Cells are fluorescent at t= 210min and OD=0.5.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Every 15 minutes we check bacterial OD and fluorescence. Cells are fluorescent at t= 210min and OD=0.5.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We decide to repeat the test to understand if we can switch off the fluorescent cells with a higher amount of glucose.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We decide to repeat the test to understand if we can switch off the fluorescent cells with a higher amount of glucose.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We will perform this test on <del class="diffchange diffchange-inline">07/08</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We will perform this test on <ins class="diffchange diffchange-inline">8th August</ins>.</div></td></tr>
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Silvia.tamarri2
http://2007.igem.org/wiki/index.php?title=Week_5&diff=18666&oldid=prev
Guido Costa at 13:28, 12 September 2007
2007-09-12T13:28:27Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/30/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/30/07'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We check <del class="diffchange diffchange-inline">bacterial </del>[[growth curve]].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We check [[growth curve]] <ins class="diffchange diffchange-inline">for bacteria containing an intermediate plasmid</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transformation for [http://partsregistry.org/Part:BBa_C0012 C0012], [http://partsregistry.org/Part:BBa_J52034 J52034] and [http://partsregistry.org/Part:BBa_B0015 B0015].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transformation for [http://partsregistry.org/Part:BBa_C0012 C0012], [http://partsregistry.org/Part:BBa_J52034 J52034] and [http://partsregistry.org/Part:BBa_B0015 B0015].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligation [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] (vector 2ul, insert 6ul) and ligation [http://partsregistry.org/Part:BBa_J04500 J04500]+ [http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)](vector 2ul, insert 10ul).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligation [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] (vector 2ul, insert 6ul) and ligation [http://partsregistry.org/Part:BBa_J04500 J04500]+ [http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)](vector 2ul, insert 10ul).</div></td></tr>
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Guido Costa
http://2007.igem.org/wiki/index.php?title=Week_5&diff=18286&oldid=prev
Francesca.bugane2 at 09:30, 10 September 2007
2007-09-10T09:30:31Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 09:30, 10 September 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)] and [http://partsregistry.org/Part:BBa_B0015 B0015]and [http://partsregistry.org/Part:BBa_J22101 J22101] and [http://partsregistry.org/Part:BBa_R0051 R0051] + [http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] run on electrophoresis gel and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)] and [http://partsregistry.org/Part:BBa_J22101 J22101] extraction;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)] and [http://partsregistry.org/Part:BBa_B0015 B0015]and [http://partsregistry.org/Part:BBa_J22101 J22101] and [http://partsregistry.org/Part:BBa_R0051 R0051] + [http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] run on electrophoresis gel and [http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)] and [http://partsregistry.org/Part:BBa_J22101 J22101] extraction;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-Glycerol stocks preparation<del class="diffchange diffchange-inline">;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-Glycerol stocks preparation<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
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Francesca.bugane2
http://2007.igem.org/wiki/index.php?title=Week_5&diff=17918&oldid=prev
Silvia.tamarri2 at 10:22, 5 September 2007
2007-09-05T10:22:55Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:22, 5 September 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/31/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/31/07'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012] , <del class="diffchange diffchange-inline">so </del>we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] <del class="diffchange diffchange-inline">colony </del>in 5ml. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012], <ins class="diffchange diffchange-inline">thus </ins>we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] <ins class="diffchange diffchange-inline">colonies </ins>in 5ml. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We go along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We go along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Miniprep of [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Miniprep of [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Digestion of </del>[http://partsregistry.org/Part:BBa_B0015 B0015] with Xba/Pst1;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_B0015 B0015] <ins class="diffchange diffchange-inline">digestion </ins>with Xba/Pst1;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">Digestion of </del>[http://partsregistry.org/Part:BBa_I52034 I52034] with Spe/Pst1;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I52034 I52034] <ins class="diffchange diffchange-inline">digestion </ins>with Spe/Pst1;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Gel extraction (we can’t see [http://partsregistry.org/Part:BBa_B0015 B0015]);</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Gel extraction (we can’t see [http://partsregistry.org/Part:BBa_B0015 B0015]);</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_R0015 R0015]+[http://partsregistry.org/Part:BBa_I13507 I13507][http://partsregistry.org/Part:BBa_I763007 (I763007)] ligation which doesn’t give colonies <del class="diffchange diffchange-inline">so </del>we transform with the other 10ul of ligation; </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_R0015 R0015]+[http://partsregistry.org/Part:BBa_I13507 I13507][http://partsregistry.org/Part:BBa_I763007 (I763007)] ligation which doesn’t give colonies <ins class="diffchange diffchange-inline">thus </ins>we transform with the other 10ul of ligation; </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051][http://partsregistry.org/Part:BBa_I763005 (I763005)]ligation which gives colonies so we inoculate in 5ml;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051][http://partsregistry.org/Part:BBa_I763005 (I763005)]ligation which gives colonies so we inoculate in 5ml;</div></td></tr>
</table>
Silvia.tamarri2
http://2007.igem.org/wiki/index.php?title=Week_5&diff=17915&oldid=prev
Silvia.tamarri2 at 10:19, 5 September 2007
2007-09-05T10:19:05Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:19, 5 September 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012] , so we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] colony in 5ml. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012] , so we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] colony in 5ml. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*We <del class="diffchange diffchange-inline">get </del>along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*We <ins class="diffchange diffchange-inline">go </ins>along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-From the glycerol stock we sprout <del class="diffchange diffchange-inline">1 ml </del>at 37°C for <del class="diffchange diffchange-inline">1 hour </del>in agitation.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-From the glycerol stock we sprout <ins class="diffchange diffchange-inline">1ml </ins>at 37°C for <ins class="diffchange diffchange-inline">1h </ins>in agitation.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Every 15 minutes we check bacterial OD and fluorescence. Cells are fluorescent at t= 210min and OD=0.5.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Every 15 minutes we check bacterial OD and fluorescence. Cells are fluorescent at t= 210min and OD=0.5.</div></td></tr>
</table>
Silvia.tamarri2
http://2007.igem.org/wiki/index.php?title=Week_5&diff=17912&oldid=prev
Silvia.tamarri2 at 10:16, 5 September 2007
2007-09-05T10:16:12Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:16, 5 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We check bacterial [[growth curve]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We check bacterial [[growth curve]].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transformation for [http://partsregistry.org/Part:BBa_C0012 C0012], [http://partsregistry.org/Part:BBa_J52034 J52034] and [http://partsregistry.org/Part:BBa_B0015 B0015].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transformation for [http://partsregistry.org/Part:BBa_C0012 C0012], [http://partsregistry.org/Part:BBa_J52034 J52034] and [http://partsregistry.org/Part:BBa_B0015 B0015].</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Ligation [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] (vector 2ul, insert 6ul) and <del class="diffchange diffchange-inline">the </del>ligation [http://partsregistry.org/Part:BBa_J04500 J04500]+ [http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)](vector 2ul, insert 10ul).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Ligation [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] (vector 2ul, insert 6ul) and ligation [http://partsregistry.org/Part:BBa_J04500 J04500]+ [http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)](vector 2ul, insert 10ul).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Silvia.tamarri2
http://2007.igem.org/wiki/index.php?title=Week_5&diff=17911&oldid=prev
Silvia.tamarri2 at 10:15, 5 September 2007
2007-09-05T10:15:33Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 10:15, 5 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We check bacterial [[growth curve]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We check bacterial [[growth curve]].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transformation for [http://partsregistry.org/Part:BBa_C0012 C0012], [http://partsregistry.org/Part:BBa_J52034 J52034] and [http://partsregistry.org/Part:BBa_B0015 B0015].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transformation for [http://partsregistry.org/Part:BBa_C0012 C0012], [http://partsregistry.org/Part:BBa_J52034 J52034] and [http://partsregistry.org/Part:BBa_B0015 B0015].</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Ligation [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] (vector <del class="diffchange diffchange-inline">2 ul</del>, insert <del class="diffchange diffchange-inline">6 ul</del>) and the ligation [http://partsregistry.org/Part:BBa_J04500 J04500]+ [http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)](vector <del class="diffchange diffchange-inline">2 ul</del>, insert <del class="diffchange diffchange-inline">10 ul</del>).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Ligation [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I13507 I13507] [http://partsregistry.org/Part:BBa_I763007 (I763007)] (vector <ins class="diffchange diffchange-inline">2ul</ins>, insert <ins class="diffchange diffchange-inline">6ul</ins>) and the ligation [http://partsregistry.org/Part:BBa_J04500 J04500]+ [http://partsregistry.org/Part:BBa_C0051 C0051] [http://partsregistry.org/Part:BBa_I763005 (I763005)](vector <ins class="diffchange diffchange-inline">2ul</ins>, insert <ins class="diffchange diffchange-inline">10ul</ins>).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 8:</td>
<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/31/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/31/07'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012] , so we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] colony in <del class="diffchange diffchange-inline">5 ml</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012] , so we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] colony in <ins class="diffchange diffchange-inline">5ml</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-From the glycerol stock we sprout 1 ml at 37°C for 1 hour in agitation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-From the glycerol stock we sprout 1 ml at 37°C for 1 hour in agitation.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-Every 15 minutes we check bacterial OD and fluorescence. Cells are fluorescent at t= <del class="diffchange diffchange-inline">210 min </del>and OD=0.5.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-Every 15 minutes we check bacterial OD and fluorescence. Cells are fluorescent at t= <ins class="diffchange diffchange-inline">210min </ins>and OD=0.5.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We decide to repeat the test to understand if we can switch off the fluorescent cells with a higher amount of glucose.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We decide to repeat the test to understand if we can switch off the fluorescent cells with a higher amount of glucose.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We will perform this test on 07/08.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We will perform this test on 07/08.</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 30:</td>
<td colspan="2" class="diff-lineno">Line 30:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Gel extraction (we can’t see [http://partsregistry.org/Part:BBa_B0015 B0015]);</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Gel extraction (we can’t see [http://partsregistry.org/Part:BBa_B0015 B0015]);</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_R0015 R0015]+[http://partsregistry.org/Part:BBa_I13507 I13507][http://partsregistry.org/Part:BBa_I763007 (I763007)] ligation which doesn’t give colonies so we transform with the other <del class="diffchange diffchange-inline">10 ul </del>of ligation; </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_R0015 R0015]+[http://partsregistry.org/Part:BBa_I13507 I13507][http://partsregistry.org/Part:BBa_I763007 (I763007)] ligation which doesn’t give colonies so we transform with the other <ins class="diffchange diffchange-inline">10ul </ins>of ligation; </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051][http://partsregistry.org/Part:BBa_I763005 (I763005)]ligation which gives colonies so we inoculate in 5ml;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_J04500 J04500]+[http://partsregistry.org/Part:BBa_C0051 C0051][http://partsregistry.org/Part:BBa_I763005 (I763005)]ligation which gives colonies so we inoculate in 5ml;</div></td></tr>
</table>
Silvia.tamarri2
http://2007.igem.org/wiki/index.php?title=Week_5&diff=17901&oldid=prev
Silvia.tamarri2 at 09:25, 5 September 2007
2007-09-05T09:25:29Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 09:25, 5 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 8:</td>
<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/31/07'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>::'''07/31/07'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012] , so we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] colony in 5 ml.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*There are no colonies for [http://partsregistry.org/Part:BBa_C0012 C0012] , so we inoculate [http://partsregistry.org/Part:BBa_I52034 I52034] and [http://partsregistry.org/Part:BBa_B0015 B0015] colony in 5 ml. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We strake on plates 30/07 ligations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*We get along with the [http://partsregistry.org/Part:BBa_J04431 J04431] fluorescence test:</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 74:</td>
<td colspan="2" class="diff-lineno">Line 74:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Glycerol stocks preparation;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Glycerol stocks preparation;</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Silvia.tamarri2