Week 9
From 2007.igem.org
(Difference between revisions)
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-we agree the position of LacI not controlled by Plac but by costitutionaly expressed as PTetR gene (R0040); | -we agree the position of LacI not controlled by Plac but by costitutionaly expressed as PTetR gene (R0040); | ||
| - | -reseach of genomic pLacI sequence to draw the primers or to order synthesis. | + | -reseach of genomic pLacI sequence to draw the primers or to order the synthesis. |
| + | |||
| + | *We transform new biobricks:E0020 (to put after CMV J52034 promoter) and R0040 (whith witch adjust LacI expression); | ||
| + | |||
| + | * We inoculate a S03520, S0100, B0034, J06550, S0103 colony in 5 ml; | ||
| + | |||
| + | * We observe that there isn't a colony to R0051+P0412(I763010) ligation, so we abandon this way. | ||
::'''08/29/07''' | ::'''08/29/07''' | ||
Revision as of 10:28, 31 August 2007
- 08/27/07
- New R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2 ul Ligasi).
- Transformation of R0051+P0412 (I763010) ligation and of new biobricks S03520, S0100, B0034, J06550, S0103.
- 08/28/07
- We hold a meeting and the topics of the day are:
-model agreed;
-we agree the position of LacI not controlled by Plac but by costitutionaly expressed as PTetR gene (R0040);
-reseach of genomic pLacI sequence to draw the primers or to order the synthesis.
- We transform new biobricks:E0020 (to put after CMV J52034 promoter) and R0040 (whith witch adjust LacI expression);
- We inoculate a S03520, S0100, B0034, J06550, S0103 colony in 5 ml;
- We observe that there isn't a colony to R0051+P0412(I763010) ligation, so we abandon this way.
- 08/29/07
- 08/30/07
- 08/31/07
