Week 9

(Difference between revisions)

Revision as of 10:17, 5 September 2007

08/27/07

New R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2ul Ligase).
We transform R0051+P0412 (I763010) ligation and new S03520, S0100, B0034, J06550, S0103 biobricks.

08/28/07

-model -LacI cloning under the pTetR R0040)promoter control;

08/29/07

Miniprep of S03520, S0100, B0034, J06550, S0103.
S03520, S0100, J06550, S0103 digestions with Xba/Pst1.
B0034 digestion with Spe/Pst1.
Run on electroforesi gel and S03520, S0100, B0034 band extraction. We get some problems with J06550 plasmid vector molecular wheigh (too heavy) and with S0103 insert molecular weight (too heavy). So, we decide to use another biobrick.
Ligation for I763012 + J06550 (I763016), I763012 + S0103 (missed in the sendbox),B0034 + J22101 (missed in the sendbox), B0034 + J04631 (I763020).
We transform the ligations.

08/30/07

There are colonies in the 07/30/07 plates.
We inoculate a E0020 and R0040 colony in 5 ml.
We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).

08/31/07

Miniprep of I763012 + J06550 (I763016), I763012+ S0103 (missed in the sandbox), B0034 +

J22101 (missed in the sandbox), B0034 + J04631 (I763020), E0020 and of R0040.

B0034 + J22101 digestion with Xba/Pst1; B0034 + J04631 (I763020) digestion with Xba/Pst1;

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 ::'''08/27/07'''
-*New [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2 ul Ligase).
+*New [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul Ligase).
-*Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation and of new biobricks [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103].
+*We transform [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation and new [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103] biobricks.