Week 9

From 2007.igem.org

(Difference between revisions)
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*[http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_J06550 J06550],  [http://partsregistry.org/Part:BBa_S0103 S0103] digestions with Xba/Pst1.
*[http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_J06550 J06550],  [http://partsregistry.org/Part:BBa_S0103 S0103] digestions with Xba/Pst1.
*[http://partsregistry.org/Part:BBa_B0034 B0034] digestion with Spe/Pst1.
*[http://partsregistry.org/Part:BBa_B0034 B0034] digestion with Spe/Pst1.
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*Run on electroforesi gel and [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034] band extraction. We get some problems with [http://partsregistry.org/Part:BBa_J06550 J06550] plasmid vector molecular weight (too heavy) and with [http://partsregistry.org/Part:BBa_S0103 S0103] insert molecular weight (too heavy). So, we decide to use another biobrick.
+
*Run on electroforesi gel and [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034] band extraction. We get some problems with [http://partsregistry.org/Part:BBa_J06550 J06550] plasmid vector molecular weight (too heavy) and with [http://partsregistry.org/Part:BBa_S0103 S0103] insert molecular weight (too heavy). Thus, we decide to use another biobrick.
*Ligation for [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] ([http://partsregistry.org/Part:BBa_I763016 I763016]), [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sendbox),[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sendbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763020 I763020]).
*Ligation for [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] ([http://partsregistry.org/Part:BBa_I763016 I763016]), [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sendbox),[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sendbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763020 I763020]).
*We transform the ligations.
*We transform the ligations.

Revision as of 10:22, 5 September 2007

08/27/07



08/28/07
  • Lab meeting on:

-model -LacI cloning under the pTetR R0040)promoter control;

08/29/07



08/30/07
  • There are colonies in the 07/30/07 plates.
  • We inoculate a E0020 and R0040 colony in 5ml.
  • We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).


08/31/07

J22101 (missed in the sandbox), B0034 + J04631 (I763020), E0020 and of R0040.

B0034 + J22101 digestion with Xba/Pst1; B0034 + J04631 (I763020) digestion with Xba/Pst1;

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