Week 9

From 2007.igem.org

(Difference between revisions)
Line 10: Line 10:
-model;
-model;
-
-LacI cloning under the pTetR [http://partsregistry.org/Part:BBa_R0040 R0040])promoter control.  
+
*-LacI cloning under the pTetR [http://partsregistry.org/Part:BBa_R0040 R0040])promoter control.  
*We transform new biobricks:[http://partsregistry.org/Part:BBa_E0020 E0020] and [http://partsregistry.org/Part:BBa_R0040 R0040]
*We transform new biobricks:[http://partsregistry.org/Part:BBa_E0020 E0020] and [http://partsregistry.org/Part:BBa_R0040 R0040]
Line 41: Line 41:
*[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] digestion with Spe/Pst1;
*[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] digestion with Spe/Pst1;
-
*[http://partsregistry.org/Part:BBa_B0034 B0034] +
+
*[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] digestion with Xba/Pst1;
-
[http://partsregistry.org/Part:BBa_J22101 J22101] digestion with Xba/Pst1;
+
*[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] digestion with Xba/Pst1;
*[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] digestion with Xba/Pst1;
*[http://partsregistry.org/Part:BBa_E0020 E0020] digestion with Xba/Pst1  
*[http://partsregistry.org/Part:BBa_E0020 E0020] digestion with Xba/Pst1  

Revision as of 09:35, 6 September 2007

08/27/07



08/28/07
  • Lab meeting on:

-model;

  • -LacI cloning under the pTetR R0040)promoter control.
08/29/07



08/30/07
  • There are colonies in the 07/30/07 plates.
  • We inoculate a E0020 and R0040 colony in 5ml.
  • We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).


08/31/07

J22101 (missed in the sandbox), B0034 + J04631 (I763020), E0020 and of R0040.


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