Week 9

From 2007.igem.org

(Difference between revisions)
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::'''08/27/07'''
::'''08/27/07'''
*New [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul Ligase).
*New [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul Ligase).
-
*We transform [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation and new [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103] biobricks.  
+
*We transform [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation and new [http://partsregistry.org/Part:BBa_S03520 S03520],[http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103] biobricks.  
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-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] with Eco/Pst1.
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] with Eco/Pst1.
* Band extraction from gel for:
* Band extraction from gel for:
-
*Miniprep of :
 
-
-[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] [http://partsregistry.org/Part:BBa_I763016 (I763016)];
 
-
 
-[http://partsregistry.org/Part:BBa_I763012 I763012]+ [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sandbox);
-[http://partsregistry.org/Part:BBa_I763012 I763012]+ [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sandbox);

Revision as of 09:51, 6 September 2007

08/27/07



08/28/07
  • Lab meeting on:

-model;

  • -LacI cloning under the pTetR R0040)promoter control.
08/29/07



08/30/07
  • There are colonies in the 07/30/07 plates.
  • We inoculate a E0020 and R0040 colony in 5ml.
  • We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).


08/31/07
  • Miniprep of :

-I763012 + J06550 (I763016);

-I763012+ S0103 (missed in the sandbox);

-B0034 + J22101 (missed in the sandbox);

-B0034 + J04631 (I763020);

-E0020 ;

-R0040.

  • Digestion for:

-I763012 + S0103 with Spe/Pst1;

-B0034 + J22101 with Xba/Pst1;

-B0034 + J04631 (I763020) with Xba/Pst1;

-E0020 with Xba/Pst1 ;

-R0040 with Spe/Pst1.

  • Control digestion for:

-I763012 + S0103 with Eco/Pst1;

-B0034 + J22101 with Eco/Pst1;

-B0034 + J04631 (I763020) with Eco/Pst1.

  • Band extraction from gel for:

-I763012+ S0103 (missed in the sandbox);

-B0034 + J22101 (missed in the sandbox);

-B0034 + J04631 (I763020);

-E0020 ;

-R0040.


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