Week 9

From 2007.igem.org

(Difference between revisions)
Line 63: Line 63:
* Band extraction from gel for:
* Band extraction from gel for:
-
-[http://partsregistry.org/Part:BBa_I763012 I763012]+ [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sandbox);
+
-[http://partsregistry.org/Part:BBa_I763012 I763012]+ [http://partsregistry.org/Part:BBa_S0103 S0103] ([http://partsregistry.org/Part:BBa_I763016 (I763016)];
-[http://partsregistry.org/Part:BBa_B0034 B0034] +   
-[http://partsregistry.org/Part:BBa_B0034 B0034] +   
Line 73: Line 73:
   
   
-[http://partsregistry.org/Part:BBa_R0040 R0040].
-[http://partsregistry.org/Part:BBa_R0040 R0040].
 +
*Ligations for
 +
 +
-
[[Bologna | Back]]
[[Bologna | Back]]

Revision as of 07:54, 7 September 2007

08/27/07



08/28/07
  • Lab meeting on:

-model;

  • -LacI cloning under the pTetR R0040)promoter control.
08/29/07



08/30/07
  • There are colonies in the 07/30/07 plates.
  • We inoculate a E0020 and R0040 colony in 5ml.
  • We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).


08/31/07
  • Miniprep of :

-I763012 + J06550

-I763012+ S0103 (I763016);

-B0034 + J22101 (missed in the sandbox);

-B0034 + J04631 (I763020);

-E0022 ;

-R0040.

  • Digestion for:

-I763012 + S0103 with Spe/Pst1;

-B0034 + J22101 with Xba/Pst1;

-B0034 + J04631 (I763020) with Xba/Pst1;

-E0022 with Xba/Pst1 ;

-R0040 with Spe/Pst1.

  • Band extraction from gel for:

-I763012+ S0103 ((I763016);

-B0034 + J22101 (missed in the sandbox);

-B0034 + J04631 (I763020);

-E0020 ;

-R0040.

  • Ligations for

-


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