Week 9
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- 08/27/07
- New R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2 ul Ligase).
- Transformation of R0051+P0412 (I763010) ligation and of new biobricks S03520, S0100, B0034, J06550, S0103.
- 08/28/07
- Lab meeting on:
-model -LacI cloning under the pTetR R0040)promoter control;
- 08/29/07
- Miniprep of S03520, S0100, B0034, J06550, S0103.
- S03520, S0100, J06550, S0103 digestions with Xba/Pst1.
- B0034 digestion with Spe/Pst1.
- Run on electroforesi gel and S03520, S0100, B0034 band extraction. We notice problems with J06550 band height (the insert is too heavy) and with S0103 band because the plasmid too heavy, however we extract insert bands for both.
- Ligation with a new protocol of I763012 + J06550 (I763016), I763012 + S0103 (missed in the sendbox),B0034 + J22101 (missed in the sendbox), B0034 + J04631 (I763020).
- We transform the ligations.
- 08/30/07
- There are colonies in the ligations.
- We inoculate ligations, E0020 and R0040 colony in 5 ml.
- We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).
- 08/31/07
J22101 (missed in the sandbox), B0034 + J04631 (I763020), E0020 and of R0040.