Week 9

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Revision as of 19:30, 7 September 2007 by Francesca.bugane2 (Talk | contribs)
08/27/07



08/28/07
  • Lab meeting on:

-model;

  • -LacI cloning under the pTetR R0040)promoter control.
08/29/07



08/30/07
  • There are colonies in the 07/30/07 plates.
  • We inoculate a E0020 and R0040 colony in 5ml.
  • We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).


08/31/07
  • Miniprep of :

-I763012 + J06550

-I763012+ S0103 (I763016);

-B0034 + J22101 (missed in the sandbox);

-B0034 + J04631 (I763020);

-E0022 ;

-R0040.

  • Digestion for:

-I763012 + S0103 with Spe/Pst1;

-B0034 + J22101 with Xba/Pst1;

-B0034 + J04631 (I763020) with Xba/Pst1;

-E0022 with Xba/Pst1 ;

-R0040 with Spe/Pst1.

  • Band extraction from gel for:

-I763012+ S0103 ((I763016);

-B0034 + J22101 (missed in the sandbox);

-B0034 + J04631 (I763020);

-E0020 ;

-R0040.

  • Ligations for

-I763016+I763020;

-R0040+S0100;

-R0040+S03520;

-I763005+I763015;

-J52034+E0022;

  • Transformations for the ligations.

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