BerkiGEM2007-AustinConstructionFile53

From 2007.igem.org

PCR AD0012F/AD0012R on K-12 strain genome (2701 bp)
Post PCR digest with (NdeI/XhoI/DpnI)

Digest petDUET (NdeI/XhoI, 5412/8, Larger)

Ligation product is I716353