NYMU Taipei/Lab Notes/2007 9 12

From 2007.igem.org

  • about PCR issue for INS_A and INS_B
    • so far we have two PCR (9/5 and 9/8)
    • Because the concentration of 9/8 is too low, we suspect that the temperature could cause the problem
    • five annealing temperatures are tested in INS_A
      • #1: 49.8
      • #2: 52.3
      • #3: 55.5
      • #c: 55.5 (control without template)
      • #4: 58.7
      • #5: 60.9
      • PCR protocol
        • template 1.0 uL
        • 10X PCR buffer 5.0 uL
        • dNTP (2.5mM), 2.0 uL
        • primer F 1.0 uL
        • primer R 1.0 uL
        • polymerase 0.5 uL
        • ddH2O 39.5 uL
        • total is 50.0 uL