From 2007.igem.org

Revision as of 17:46, 29 May 2007 (view source) Dbellin (Talk | contribs) ← Older edit		Revision as of 17:47, 29 May 2007 (view source) Dbellin (Talk | contribs) (→Headline text) Newer edit →
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	1.  Do primer order		1.  Do primer order
	2.  Start conjugation practice		2.  Start conjugation practice
-	3.  Confirm restriction enzymes, ligases, plasmids,	+	3.  Confirm restriction enzymes, ligases
		+	4.  Order confirmed/needed materials
		+	5.  Team Revew Meeting

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Revision as of 17:47, 29 May 2007

Headline text

Welcome to the wiki for Boston University's iGEM 2007 Team!

Our project is aimed at increasing current production from Shewanella Oneidensis by directed evolution of global transcription factors Our plan so far:

1. Mutate global transcription factors

  a.  Design primers for amplification
  b.  Perform error-prone PCR

2. Transform mutated genes into E. coli

  a.  Choose plasmids
  b.  Restriction enzyme digestion
  c.  Ligation
  d.  Transformation into E. coli
  

3. Conjugate E. coli with Shewanella

4. Screen/select for increased current production due to mutations

Week's Goals:

Wednesday 5/30 1. Get all protocols 2. Identify materials/prepare order 3. Design Primers 4. Learn about budget/POs

Thursday 5/31 1. Do primer order 2. Start conjugation practice 3. Confirm restriction enzymes, ligases 4. Order confirmed/needed materials 5. Team Revew Meeting