USTC/Logic-Gate Promoters

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== Cis-acting Bio-Logic Gates ==
== Cis-acting Bio-Logic Gates ==
-
In natural cells, combinational logic computation can be carried out by cis-acting elements []. Theoretically, dual repressors interacting on two adjacent operators can generate complex logic function as NAND, NOT and NOT []. But seldom of the parameters of these models have been measured, and practical artificial logic promoters are hard to made because of the lack of appropriate inputs. In this project, we simplify these models to reduce the number of parameters, use artificial high-specific repressors based-on Lac repressor as inputs, predict possible pattern of logic promoters, construct and test them experimentally, to attempt to find a systematical way to construct cis-acting bio-logic promoters. As the results, a piece of DNA about 60 200bp (2.0nm in width, 20-70nm in length, similar to transistors in present VSLI in size[], sometimes even smaller) is able to be built up and to act as a logic gate.
+
In natural cells, combinational logic computation can be carried out by cis-acting elements [[USTC/Logic-Gate_Promoters#References|[4]]]. Theoretically, dual repressors interacting on two adjacent operators can generate complex logic function as NAND, NOT and NOT [[USTC/Logic-Gate_Promoters#References|[1,2,3]]]. However, seldom of the parameters of these models has been measured, and practical artificial logic promoters are hard to make because of the lack of appropriate inputs. In this project, we simplify these models to reduce the number of parameters, use artificial high-specific repressors based-on Lac repressor [[USTC/Logic-Gate_Promoters#References|[8]]] to serve as inputs, predict possible patterns of logic promoters, construct and test them experimentally, all to attempt to find a systematical way to construct cis-acting bio-logic promoters. As a result, a piece of DNA about 60 - 200bp is able to be built up and to act as a logic gate.
 +
 
 +
=== Advatanges of Cis-acting Bio-Logic Gates ===
 +
# Work <i>in vivo</i> and can be genetically inherited
 +
# Can be systematically built up according to several patterns
 +
# Small in scale
 +
#* About 2.0nm in width, 20 - 70nm in length, similar to transistors in present VSLI in size [[USTC/Logic-Gate_Promoters#References|[12]]], sometimes even smaller
 +
# Can be cascaded to implement any complex combinational logic computation
 +
#* And is also able to form sequential circuit
== Repression Model ==
== Repression Model ==
-
Lacramioara Bintu et al. have reported a simple thermodynamic model which can quantitatively describe promoter activity under one or more regulator factors. In this project, we focus on fold-change under one or two repressors, which means the ratio of promoter activity in the absence and presence of repressor. For a weak promoter, the fold-change can be written approximately as a function of repressor concentrations, inter-operator distances, repressor–operator affinity and repressor-repressor interactions.
+
[[Image:USTC_RepressionModel.png|thumb|right|300px|'''Figure 1'''  (a) Sketch map of solo-repression. (b) Sketch map of co-repression.]]
-
For a promoter containing a single operator site shown in Figure 1(a), the fold-change is given as:
+
Lacramioara Bintu et al. have reported a simple thermodynamic model which can quantify promoter activity under one or more regulatory factors [[USTC/Logic-Gate_Promoters#References|[1,2]]]. In this project, we focus on the multiple changes of promoter activity under the existence of one or two repressors. For a weak promoter, the multiple of its change can be approximately described as a function of different repressor concentrations, inter-operator distances, repressor–operator affinity and repressor-repressor interactions.
-
TODO
+
For a promoter containing a single operator site shown in Figure 1(a), the promoter activity under <i>R</i> repressor molecules <i>A(R)</i> is:
-
For a promoter containing two different operators, of which the relative repressors may be able to interact with each other shown in Figure 1(b), the fold-change is given as:
+
[[Image:USTC_RepressionModel_FC_Solo.png|center]]
-
TODO
+
Note that <i>A(0)</i> is the promoter activity without repression; <i>&rho;(P)</i> is the solo-repression coefficient of the operator at the position <i>P</i>; <i>&Delta;&epsilon;(O)</i> is the difference of binding energy of operator <i>O</i> on specific sites to non-specific sites; <i>N<sub>NS</sub></i> is the number of non-specific sites; and <i>K<sub>B</sub></i> means the Boltzmann constant, <i>T</i> is the temperature.
-
Concerning a NOT gate which works under approximate equal high and low repressor concentration, R<sub>low</sub>=0 and R<sub>high</sub>=R<sub>H</sub>, its performance can be expressed simply as NOT factor in a non-dimension form:
+
For a promoter containing two different operators, of which the relative repressors may be able to interact with each other shown in Figure 1(b), the promoter activity under combinations of two repressors, R<sub>A</sub> and R<sub>B</sub>, is given as:
-
TODO
+
[[Image:USTC_RepressionModel_FC_Co.png|center]]
-
In the same way, NAND factor and NOR factor are:
+
Where <i>&omega;(P<sub>A</sub>, P<sub>B</sub>)</i> is the co-repression coefficient when O<sub>A</sub> is located at <i>P<sub>A</sub></i>, and O<sub>B</sub> at <i>P<sub>B</sub></i>.
-
TODO
+
Concerning a NOT gate which works under approximately equal high or low repressor concentration, R<sub>low</sub>=0 and R<sub>high</sub>=R<sub>H</sub>, we assessed its performance by giving it a score:
 +
[[Image:USTC_RepressionModel_NOT_Score.png|center]]
 +
 +
In the same way, NAND score and NOR score are:
 +
 +
[[Image:USTC_RepressionModel_NAND_Score.png|center]]
 +
 +
[[Image:USTC_RepressionModel_NOR_Score.png|center]]
 +
 +
In the situation with a fixed combination of two repressors, R<sub>A</sub> and R<sub>B</sub>, and approximately equal high or low repressor concentration, the logic performance of a promoter is a function of inter-operator distances, repressor–operator affinity and repressor-repressor interactions. By adjusting these parameters, it is possible to find out well-performing bio-logic promoters.
 +
 +
 +
 +
 +
----
== Schemes of Bio-Logic Promoters ==
== Schemes of Bio-Logic Promoters ==
 +
 +
Dozens of potential bio-logic patterns were experimentally synthesized and tested in solo-repression or co-repression test-bench. Some representative ones are shown and commented as following.
 +
 +
{| border="1"
 +
|-
 +
|align="center"| '''Scheme'''
 +
|align="center"| '''Test-environment'''
 +
|align="center"| '''Results'''
 +
|align="center"| '''Comments'''
 +
|-
 +
| [[Image:USTC_NANDv1.png|380px]]
 +
|align="center"| [[Image:USTC_RM_LacI_LRLa.png|92px]]
 +
|align="center"| [[Image:USTC_NANDv1_Data.png|93px]]
 +
| [[USTC/Interference between LacI and LRLa|Interference between inputs]]
 +
|-
 +
| [[Image:USTC_NORv1.png|380px]]
 +
|align="center"| [[Image:USTC_RM_LacI_LRLa.png|92px]]
 +
|align="center"| [[Image:USTC_NORv1_Data.png|93px]]
 +
| [[USTC/Interference between LacI and LRLa|Interference between inputs]]
 +
|-
 +
| [[Image:USTC_NOTv1.png|380px]]
 +
|align="center"| [[Image:USTC_RM_LacI.png|64px]]
 +
|align="center"| [[Image:USTC_NOTv1_Data.png|93px]]
 +
| <font color="red">Works</font>
 +
|-
 +
| [[Image:USTC_NANDv2a.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_ARL2A0203.png|92px]]
 +
|align="center"| [[Image:USTC_NANDv2a_Data.png|93px]]
 +
| <font color="orange">Works</font><BR>[[USTC/OperatorPosition|But with slight downstream repression]]
 +
|-
 +
| [[Image:USTC_NANDv2b.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_ARL2A0203.png|92px]]
 +
|align="center"| [[USTC/FailureOfNANDv2b|Failed in<BR>X-gal Assay]]
 +
| [[USTC/OperatorComposition|"Ox7" kind of operators are too weak]]
 +
|-
 +
| [[Image:USTC_NORv1.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_ARL2A0203.png|92px]]
 +
|align="center"| [[Image:USTC_NORv1_Data2.png|93px]]
 +
| [[USTC/OperatorPosition|Inefficacy of upstream operator]]
 +
|-
 +
| [[Image:USTC_NANDv3a.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_ARL2A0203.png|92px]]
 +
|align="center"| [[Image:USTC_NANDv3a_Data.png|93px]]
 +
| [[USTC/InterOperatorDistance|Co-repression is too weak]]<BR>[[USTC/OperatorPosition|Downstream solo-repression is to strong]]
 +
|-
 +
| [[Image:USTC_NANDv3b.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_ARL2A0203.png|92px]]
 +
|align="center"| [[Image:USTC_NANDv3b_Data.png|93px]]
 +
| <font color="red">Works</font>
 +
|-
 +
| [[Image:USTC_NORv2.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_ARL2A0203.png|92px]]
 +
|align="center"| [[Image:USTC_NORv2_Data.png|93px]]
 +
| [[USTC/OperatorPosition|Inefficacy of upstream operator]]
 +
|-
 +
| [[Image:USTC_NANDv4.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_ARL2A0203.png|92px]]
 +
|align="center"| [[Image:USTC_NANDv4_Data.png|93px]]
 +
| [[USTC/HybridOperator|Hybrid operator does not work as expected]]
 +
|-
 +
| [[Image:USTC_NORv3.png|380px]]
 +
|align="center"| [[Image:USTC_RM_ARL4A0604_LacI.png|92px]]
 +
|align="center"| [[Image:USTC_NORv3_Data.png|93px]]
 +
| <font color="red">Works</font><BR>[[USTC/CoRepressedOperator|With a request of co-operator]]
 +
|-
 +
|}
 +
 +
 +
 +
 +
----
 +
 +
== Valuable Experiences for Bio-Logic Promoters ==
 +
 +
=== Factors of Gate-Performances ===
 +
The level of repression <i>in vivo</i> is determined by several factors . Because both 'strong' operator and 'weak' operator are required for our system, we systematically tested the effects by the two primary factors: [[USTC/OperatorComposition|'''composition''']] and  [[USTC/OperatorPosition|'''position''']].The proper distance between two operators is also necessary for NOR and NAND gates, and data has been [[USTC/InterOperatorDistance|'''reported''']].
 +
 +
=== Hybrid Operator and Dual-Repressed Operator ===
 +
Based on the data of [[USTC/OperatorComposition|'''operators' composition''']], two ideas have been proposed and attempted to realize:
 +
* [[USTC/HybridOperator|'''Crossbreeding two specific operators''']] may be able to carry out new functions.
 +
* A specific operator repressed by two or more different repressors can be used as [[USTC/DualRepressedOperator|'''a model for NOR gate''']].
 +
 +
 +
== Repression Assay ==
== Repression Assay ==
Line 30: Line 136:
[[Image:USTC_PCRBuilding.png|thumb|400px|right|'''Figure ''' PCR Building]]
[[Image:USTC_PCRBuilding.png|thumb|400px|right|'''Figure ''' PCR Building]]
-
Firstly, we extend both sides of the conservative region for transcriptional initiation of PlacUV5[], including -35 box,-10 box and +1 starting point, with two non-sense sequence selected from random groups. The product is named as P_template1 as it is the template for the promoter family. These two non-sense sequence have three main characters:
+
Firstly, we extend both sides of the conservative region for transcriptional initiation [[USTC/Logic-Gate_Promoters#References|[9]]] of PlacUV5 [[USTC/Logic-Gate_Promoters#References|[7]]], including -35 box,-10 box and +1 starting point, with two non-sense sequence selected from random groups. The product is named as P_template1 as it is the template for the promoter family. These two non-sense sequence have three main characters:
# They will never include the restriction enzyme cutting sites that will be involved in the whole study;
# They will never include the restriction enzyme cutting sites that will be involved in the whole study;
# They will never include the recognition sites of RNA Polymerases and those of either of the two repressors;
# They will never include the recognition sites of RNA Polymerases and those of either of the two repressors;
Line 37: Line 143:
Secondly, another group of primers, of which the elongation region at 5’ end may contain a unique operator sequence or each, is applied at both ends of P_template1, equipping us with an according group of promoters with complete structures. These promoters can include variant operator sequences at different position in flank of the conservative region.
Secondly, another group of primers, of which the elongation region at 5’ end may contain a unique operator sequence or each, is applied at both ends of P_template1, equipping us with an according group of promoters with complete structures. These promoters can include variant operator sequences at different position in flank of the conservative region.
-
Then the promoter fragments are digested with XbaI and BamHI and cloned into repression-reporter plasmid, which contains <i>lacZ</i> alpha fragment and <i>gfp</i> under the promoter insertion site.
+
Then the promoter fragments are digested with XbaI and BamHI and loaded into repression-reporter plasmid, which contains <i>lacZ</i> alpha fragment and <i>gfp</i> under the promoter insertion site.
 +
 
 +
All the members of the our promoter family are named according to [[USTC/NamingRules|'''a uniform rule''']].
 +
 
=== Solo-Repression Assay ===
=== Solo-Repression Assay ===
[[Image:SoloRepressionAssay.png|thumb|right|400px|'''Figure''' Solo-Repression]]
[[Image:SoloRepressionAssay.png|thumb|right|400px|'''Figure''' Solo-Repression]]
 +
 +
Two plasmids are used in solo-repression assay. First, a plasmid constitutively expressing a specific repressor is transformed into Top10. Then the promoters to be tested, which contain variant operator compositions and positions, are transformed into the strains got in the first step and then selected through double resistance.
<BR clear="both">
<BR clear="both">
 +
=== Co-Repression Assay ===
=== Co-Repression Assay ===
-
[[Image:USTC_CoRepressionAssay.png|thumb|right|400px|'''Figure''' Co-Repression]]
+
Promoters to be tested are loaded into double-reporter plasmid and then transformed into the four test strains (CR00, CR01, CR10, CR11). By reading the color of the colonies on plates with X-Gal, and by testing the fluorescence intensity under a fluorescence microscope, we can get the solo-repression and co-repression effects of the two repressors on specific promoters.
 +
[[Image:USTC_CoRepressionAssay.png|thumb|300px|'''Figure''' Co-Repression Assay]]
 +
 
 +
{| border="1"
 +
|-
 +
|align="center"|'''Genotype'''
 +
|align="center"|'''Character'''
 +
|align="center"|'''Name'''
 +
|-
 +
|Top10/pT-TERM
 +
|So not express any repressors
 +
|align="center"|CR00
 +
|-
 +
|Top10/pT-ARL4A0604
 +
|Constitutively express ARL4A0604
 +
|align="center"|CR01
 +
|-
 +
|Top10/pT-ARL2A0203
 +
|Constitutively express ARL2A0203
 +
|align="center"|CR10
 +
|-
 +
|Top10/pTet-ARL4A0604-ARL2A203
 +
|Constitutively express ARL4A0604 and ARL2A0203
 +
|align="center"|CR11
 +
|}
<BR clear="both">
<BR clear="both">
 +
 +
 +
 +
 +
----
== Final Results ==
== Final Results ==
Line 56: Line 197:
| [[Image:USTC_BestNOT.png|thumb|200px|Best NOT]]
| [[Image:USTC_BestNOT.png|thumb|200px|Best NOT]]
|}
|}
 +
 +
 +
=== Suggested Patterns ===
 +
[[Image:USTC_BestSchemes.png|thumb|right|300px|'''Figure 5''' Suggested patterns for NOT, NAND and NOR gates.]]
 +
 +
'''NAND'''<BR>
 +
A NAND Gate requires that two solo-repressions should be weak, and co-repression should be strong. We choose +83.5 to put the upstream operator, to avoid the uncertain activator regions. Another weak operator is fixed at the +66.5 site. The relative distance between the two operators is 150, indicating a strong co-repression.
 +
 +
'''NOR'''<BR>
 +
We expected to find a NOR gate with two different operators around the conservative region of a promoter. However, there is not any available repressor binding site in the upstream of the conservative region based on the observed effect of operator positions. At present only the dual-repression pattern works well as NOR gate, but it brings us a limitation in wires selection when assembled into the whole system.
 +
 +
'''NOT'''<BR>
 +
The NOT gate is quite simple, containing only one operator of reverse symmetric structure at the +10.5 site.
<BR clear="both">
<BR clear="both">
 +
 +
 +
 +
 +
----
== References ==
== References ==
 +
 +
1. Bintu, L.; Buchler, N. E.; Garcia, H. G.; Gerland, U.; Hwa, T.; Kondev, J.; Kuhlman, T. & Phillips, R. (2005), 'Transcriptional regulation by the numbers: applications.', <i>Curr Opin Genet Dev</i> 15(2), 125--135.
 +
 +
2. Bintu, L.; Buchler, N. E.; Garcia, H. G.; Gerland, U.; Hwa, T.; Kondev, J. & Phillips, R. (2005), 'Transcriptional regulation by the numbers: models.', <i>Curr Opin Genet Dev</i> 15(2), 116--124.
 +
 +
3. Buchler, N. E.; Gerland, U. & Hwa, T. (2003), 'On schemes of combinatorial transcription logic.', <i>PNAS</i> 100(9), 5136--5141.
 +
 +
4. Davidson, E. H.; Rast, J. P.; Oliveri, P.; Ransick, A.; Calestani, C.; Yuh, C.; Minokawa, T.; Amore, G.; Hinman, V.; Arenas-Mena, C.; Otim, O.; Brown, C. T.; Livi, C. B.; Lee, P. Y.; Revilla, R.; Rust, A. G.; jun Pan, Z.; Schilstra, M. J.; Clarke, P. J. C.; Arnone, M. I.; Rowen, L.; Cameron, R. A.; McClay, D. R.; Hood, L. & Bolouri, H. (2002), A genomic regulatory network for development., <i>Science</i> 295(5560), 1669--1678.
 +
 +
5. Elledge, S. J. & Davis, R. W. (1989), 'Position and density effects on repression by stationary and mobile DNA-binding proteins.', <i>Genes Dev</i> 3(2), 185--197.
 +
 +
6. Kalodimos, C. G.; Bonvin, A. M. J. J.; Salinas, R. K.; Wechselberger, R.; Boelens, R. & Kaptein, R. (2002), 'Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain.', <i>EMBO J</i> 21(12), 2866--2876.
 +
 +
7. Lanzer, M. & Bujard, H. (1988), 'Promoters largely determine the efficiency of repressor action.', <i>PNAS</i> 85(23), 8973--8977.
 +
 +
8. Lewis, M. (2005), 'The lac repressor.', <i>C R Biol</i> 328(6), 521--548.
 +
 +
9. Rojo, F. (1999), 'Repression of transcription initiation in bacteria.', <i>J Bacteriol</i> 181(10), 2987--2991.
 +
 +
10. Saiz, L. & Vilar, J. M. G. (2006), 'DNA looping: the consequences and its control.', <i>Curr Opin Struct Biol</i> 16(3), 344--350.
 +
 +
11. Sheridan, S. D.; Opel, M. L. & Hatfield, G. W. (2001), 'Activation and repression of transcription initiation by a distant DNA structural transition.', <i>Mol Microbiol</i> 40(3), 684--690.
 +
 +
12. [http://cnse.albany.edu/News/index.cfm?step=show_detail&NewsID=424 Semiconductor International: 45 to 32 nm: Another Evolutionary Transition.]
 +
 +
 +
<br>

Latest revision as of 03:15, 27 October 2007

Contents

Cis-acting Bio-Logic Gates

In natural cells, combinational logic computation can be carried out by cis-acting elements [4]. Theoretically, dual repressors interacting on two adjacent operators can generate complex logic function as NAND, NOT and NOT [1,2,3]. However, seldom of the parameters of these models has been measured, and practical artificial logic promoters are hard to make because of the lack of appropriate inputs. In this project, we simplify these models to reduce the number of parameters, use artificial high-specific repressors based-on Lac repressor [8] to serve as inputs, predict possible patterns of logic promoters, construct and test them experimentally, all to attempt to find a systematical way to construct cis-acting bio-logic promoters. As a result, a piece of DNA about 60 - 200bp is able to be built up and to act as a logic gate.

Advatanges of Cis-acting Bio-Logic Gates

  1. Work in vivo and can be genetically inherited
  2. Can be systematically built up according to several patterns
  3. Small in scale
    • About 2.0nm in width, 20 - 70nm in length, similar to transistors in present VSLI in size [12], sometimes even smaller
  4. Can be cascaded to implement any complex combinational logic computation
    • And is also able to form sequential circuit

Repression Model

Figure 1 (a) Sketch map of solo-repression. (b) Sketch map of co-repression.

Lacramioara Bintu et al. have reported a simple thermodynamic model which can quantify promoter activity under one or more regulatory factors [1,2]. In this project, we focus on the multiple changes of promoter activity under the existence of one or two repressors. For a weak promoter, the multiple of its change can be approximately described as a function of different repressor concentrations, inter-operator distances, repressor–operator affinity and repressor-repressor interactions.

For a promoter containing a single operator site shown in Figure 1(a), the promoter activity under R repressor molecules A(R) is:

USTC RepressionModel FC Solo.png

Note that A(0) is the promoter activity without repression; ρ(P) is the solo-repression coefficient of the operator at the position P; Δε(O) is the difference of binding energy of operator O on specific sites to non-specific sites; NNS is the number of non-specific sites; and KB means the Boltzmann constant, T is the temperature.

For a promoter containing two different operators, of which the relative repressors may be able to interact with each other shown in Figure 1(b), the promoter activity under combinations of two repressors, RA and RB, is given as:

USTC RepressionModel FC Co.png

Where ω(PA, PB) is the co-repression coefficient when OA is located at PA, and OB at PB.

Concerning a NOT gate which works under approximately equal high or low repressor concentration, Rlow=0 and Rhigh=RH, we assessed its performance by giving it a score:

USTC RepressionModel NOT Score.png

In the same way, NAND score and NOR score are:

USTC RepressionModel NAND Score.png
USTC RepressionModel NOR Score.png

In the situation with a fixed combination of two repressors, RA and RB, and approximately equal high or low repressor concentration, the logic performance of a promoter is a function of inter-operator distances, repressor–operator affinity and repressor-repressor interactions. By adjusting these parameters, it is possible to find out well-performing bio-logic promoters.




Schemes of Bio-Logic Promoters

Dozens of potential bio-logic patterns were experimentally synthesized and tested in solo-repression or co-repression test-bench. Some representative ones are shown and commented as following.

Scheme Test-environment Results Comments
USTC NANDv1.png USTC RM LacI LRLa.png USTC NANDv1 Data.png Interference between inputs
USTC NORv1.png USTC RM LacI LRLa.png USTC NORv1 Data.png Interference between inputs
USTC NOTv1.png USTC RM LacI.png USTC NOTv1 Data.png Works
USTC NANDv2a.png USTC RM ARL4A0604 ARL2A0203.png USTC NANDv2a Data.png Works
But with slight downstream repression
USTC NANDv2b.png USTC RM ARL4A0604 ARL2A0203.png Failed in
X-gal Assay
"Ox7" kind of operators are too weak
USTC NORv1.png USTC RM ARL4A0604 ARL2A0203.png USTC NORv1 Data2.png Inefficacy of upstream operator
USTC NANDv3a.png USTC RM ARL4A0604 ARL2A0203.png USTC NANDv3a Data.png Co-repression is too weak
Downstream solo-repression is to strong
USTC NANDv3b.png USTC RM ARL4A0604 ARL2A0203.png USTC NANDv3b Data.png Works
USTC NORv2.png USTC RM ARL4A0604 ARL2A0203.png USTC NORv2 Data.png Inefficacy of upstream operator
USTC NANDv4.png USTC RM ARL4A0604 ARL2A0203.png USTC NANDv4 Data.png Hybrid operator does not work as expected
USTC NORv3.png USTC RM ARL4A0604 LacI.png USTC NORv3 Data.png Works
With a request of co-operator




Valuable Experiences for Bio-Logic Promoters

Factors of Gate-Performances

The level of repression in vivo is determined by several factors . Because both 'strong' operator and 'weak' operator are required for our system, we systematically tested the effects by the two primary factors: composition and position.The proper distance between two operators is also necessary for NOR and NAND gates, and data has been reported.

Hybrid Operator and Dual-Repressed Operator

Based on the data of operators' composition, two ideas have been proposed and attempted to realize:



Repression Assay

Build Up Promoter Family

Figure PCR Building

Firstly, we extend both sides of the conservative region for transcriptional initiation [9] of PlacUV5 [7], including -35 box,-10 box and +1 starting point, with two non-sense sequence selected from random groups. The product is named as P_template1 as it is the template for the promoter family. These two non-sense sequence have three main characters:

  1. They will never include the restriction enzyme cutting sites that will be involved in the whole study;
  2. They will never include the recognition sites of RNA Polymerases and those of either of the two repressors;
  3. They will never present in complicated structures.

Secondly, another group of primers, of which the elongation region at 5’ end may contain a unique operator sequence or each, is applied at both ends of P_template1, equipping us with an according group of promoters with complete structures. These promoters can include variant operator sequences at different position in flank of the conservative region.

Then the promoter fragments are digested with XbaI and BamHI and loaded into repression-reporter plasmid, which contains lacZ alpha fragment and gfp under the promoter insertion site.

All the members of the our promoter family are named according to a uniform rule.


Solo-Repression Assay

Figure Solo-Repression

Two plasmids are used in solo-repression assay. First, a plasmid constitutively expressing a specific repressor is transformed into Top10. Then the promoters to be tested, which contain variant operator compositions and positions, are transformed into the strains got in the first step and then selected through double resistance.


Co-Repression Assay

Promoters to be tested are loaded into double-reporter plasmid and then transformed into the four test strains (CR00, CR01, CR10, CR11). By reading the color of the colonies on plates with X-Gal, and by testing the fluorescence intensity under a fluorescence microscope, we can get the solo-repression and co-repression effects of the two repressors on specific promoters.

Figure Co-Repression Assay
Genotype Character Name
Top10/pT-TERM So not express any repressors CR00
Top10/pT-ARL4A0604 Constitutively express ARL4A0604 CR01
Top10/pT-ARL2A0203 Constitutively express ARL2A0203 CR10
Top10/pTet-ARL4A0604-ARL2A203 Constitutively express ARL4A0604 and ARL2A0203 CR11





Final Results

Best NAND
Best NOR
Best NOT


Suggested Patterns

Figure 5 Suggested patterns for NOT, NAND and NOR gates.

NAND
A NAND Gate requires that two solo-repressions should be weak, and co-repression should be strong. We choose +83.5 to put the upstream operator, to avoid the uncertain activator regions. Another weak operator is fixed at the +66.5 site. The relative distance between the two operators is 150, indicating a strong co-repression.

NOR
We expected to find a NOR gate with two different operators around the conservative region of a promoter. However, there is not any available repressor binding site in the upstream of the conservative region based on the observed effect of operator positions. At present only the dual-repression pattern works well as NOR gate, but it brings us a limitation in wires selection when assembled into the whole system.

NOT
The NOT gate is quite simple, containing only one operator of reverse symmetric structure at the +10.5 site.





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