Wisconsin/PCR

From 2007.igem.org

(Difference between revisions)
(Basic Non-Sequencing PCR)
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Run on Ansari PCR machine CDC-02
Run on Ansari PCR machine CDC-02
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===PCR Purification===
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Follow instruction in the [http://www1.qiagen.com/literature/protocols/QIAquickSpin.aspx QIAquick Spin Handbook]

Revision as of 19:39, 4 July 2007

Basic Non-Sequencing PCR

  • 38.5uL H2O
  • 5uL 10x ExTaq buffer
  • 1uL 10mM dNTPs
  • 1uL Forward Primer
  • 1uL Reverse Primer
  • 1uL Template
  • 0.5uL ExTaq (add this last)
  • 2uL MgCl2 25mM

Run on Ansari PCR machine CDC-02

PCR Purification

Follow instruction in the [http://www1.qiagen.com/literature/protocols/QIAquickSpin.aspx QIAquick Spin Handbook]