McGill/Team 1: Fluorescence Complementation
From 2007.igem.org
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| - | # Transformation of Cells | + | # Transformation of Cells: |
## Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each) | ## Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each) | ||
## Used 400uL of LB for each vial | ## Used 400uL of LB for each vial | ||
Revision as of 14:48, 17 May 2007
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Contents |
May 2007
May 14
- Made the same as May 15, but did not use Type 1 (milliQ) H2O, so the solutions were discarded.
May 15
- Made 1M CaCl2, and glycerol/CaCl2 solutions
- 1M CaCl2 Solution: 100mL was made
- CaCl2 Dihydrate = 146.986 g/mol
- 100mL of 1M CaCl2 requires 14.699g CaCl2 Dihydrate
- Glycerol/CaCl2 Solution: 30mL was made
- 5.00mL of 60% glycerol solution
- 3.00mL of 1M CaCl2
- 22.00mL of Type 1 (milliQ) H2O
- 1M CaCl2 Solution: 100mL was made
May 16
- Transformation of Cells:
- Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each)
- Used 400uL of LB for each vial
- Incubated for 1 hour in shaker at 37C, then incubate overnight at 37C
