Boston University/Project Progress
From 2007.igem.org
(Difference between revisions)
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==What We've Accomplished== | ==What We've Accomplished== | ||
| - | [[ | + | [[Boston_University/Plasmid Choice | Plasmid Choice]] |
| - | [[ | + | [[Boston_University/Restriction Choice | Restriction Enzyme Choice]] |
| - | [[ | + | [[Boston_University/Primer Design | Primer Design]] |
| - | [[ | + | [[Boston_University/Shewy Competence and Transformation| Making Shewanella Competent and Transforming Plasmid]] |
== Materials We Need == | == Materials We Need == | ||
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== Protocols == | == Protocols == | ||
| - | [[ | + | [[Boston_University/Heat Shock Protocol | Calcium Chloride/Heat Shock Plasmid Transformations Protocol]] |
| - | [[ | + | [[Boston_University/Filter Conjugation Protocol | Filter Cojugation Protocol]] |
| - | [[ | + | [[Boston_University/TOPO Protocol | pTrcHis TOPO TA Expression Kit Cloning Protocol]] |
| - | [[ | + | [[Boston_University/NEBCutter Protocl| Using NEBCutter for checking specific restriction enzymes against a sequence]] |
| - | [[ | + | [[Boston_University/Gel Protocol | Making an Electrophoresis Gel]] |
| - | [[ | + | [[Boston_University/Zymo Protocol | Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB |
]] | ]] | ||
Revision as of 16:27, 9 July 2007
Contents |
What We've Accomplished
Making Shewanella Competent and Transforming Plasmid
Materials We Need
Error-Prone PCR: From CAB(?)
Ligases: Need to Buy
Short-Term To-Do List
EDIT THE WIIIIKIIII
Protocols
Calcium Chloride/Heat Shock Plasmid Transformations Protocol
pTrcHis TOPO TA Expression Kit Cloning Protocol
Using NEBCutter for checking specific restriction enzymes against a sequence
Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB
