Boston University/Gel Protocol

< Boston University(Difference between revisions)

Latest revision as of 15:04, 23 July 2007

Take 60ml TAE 1X and add 0.9g agarose to make a 1.5% agarose gel.
Microwave for about 1-1.5 minutes and shake after every 20 seconds. Make SURE that all the agarose is COMPLETELY dissolved (the solution should be clear).
Let the solution cool by placing the flask under running tap water for about a minute to a minute and a half. No water should get inside the flask. Make sure that you don't cool the gel flask too much otherwise it will solidify.
Add 1ul of Etbr and swirl around.
Pour it on the gel-plate with a fork already in it.

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Load 10ul of diluted ladder in a lane.

The Fisher 1/40loading dye should have a final volume 1/5 in the overall solution.

@@ Line 1: / Line 1: @@
 == Making Electrophoresis Gel ==
-# Take 60ml TAE 1X and add 0.6g agarose.
+# Take 60ml TAE 1X and add 0.9g agarose to make a 1.5% agarose gel.
-# Microwave for about 45 seconds.
+# Microwave for about 1-1.5 minutes and shake after every 20 seconds. Make SURE that all the agarose is COMPLETELY dissolved (the solution should be clear).
-# Let it cool until its cool enough so you can touch it for an extended period of time.
+# Let the solution cool by placing the flask under running tap water for about a minute to a minute and a half. No water should get inside the flask. Make sure that you don't cool the gel flask too much otherwise it will solidify.
-# Add 4ul of Etbr and pour it on the gel-plate.
+# Add 1ul of Etbr and swirl around.
+# Pour it on the gel-plate with a fork already in it.
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@@ Line 10: / Line 11: @@
 Load 10ul of diluted ladder in a lane.
-The 1/40loading dye should have a final volume 1/5 in the overall solution .
+The Fisher 1/40loading dye should have a final volume 1/5 in the overall solution.
 [[Boston_University/Protocols | Back]]