Boston University/Plasmid Customization

From 2007.igem.org

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(→Verifying the presence of the lacZ promoter in the plasmid pJQ200)
(→Verifying the presence of the lacZ promoter in the plasmid pJQ200)
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==Excising the sacB gene from plasmid pJQ200==
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:'''PCR amplification of lacZ'''
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::::TEXT
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[[Image:BU_labeled_digested_pjq200_7-19-2007.jpg]]
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[[Image:BU_labeled_pjq200_sacb_digestion_7-17.jpg]]
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==Verifying the presence of the lacZ promoter in the plasmid pJQ200==
==Verifying the presence of the lacZ promoter in the plasmid pJQ200==
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[[Image:BU_labeled_lacz_PCR_7-19-2007.jpg]]
[[Image:BU_labeled_lacz_PCR_7-19-2007.jpg]]
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[[Image:BU_labeled_digested_pjq200_7-19-2007.jpg]]
 
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[[Image:BU_labeled_lacz_PCR_7-19-2007.jpg]]
 
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[[Image:BU_labeled_pjq200_sacb_digestion_7-17.jpg]]
 

Revision as of 18:13, 23 July 2007

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ





TEXT






BU labeled digested pjq200 7-19-2007.jpg

BU labeled pjq200 sacb digestion 7-17.jpg

Verifying the presence of the lacZ promoter in the plasmid pJQ200

PCR amplification of lacZ





TEXT






BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg

Retrieved from "http://2007.igem.org/wiki/index.php/Boston_University/Plasmid_Customization"