Calgary/testing

From 2007.igem.org

(Difference between revisions)

Revision as of 05:55, 22 October 2007

I) Colony PCR test...

  • Design universal primers (valid for any biobrick out of any plasmid)
  • Develop massive amounts
  • Gel to test

II) Test Plasmids --> always grow up 5ml of overnight

  • Plasmid Prep --> isolating plasmid out of the cell
  • Sigma Plasmid Prep protocol