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Boston University/Plasmid Customization
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Boston University
Revision as of 14:46, 24 July 2007 by
Linger
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Overview
Plasmid pJQ200
Excising the sacB gene from plasmid pJQ200
PCR amplification of lacZ
Verifying the presence of the lacZ promoter in the plasmid pJQ200
Double digestion of pJQ200 with EcoRI and HindIII