Berkeley LBL/MimiNotebook

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('''Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD''' (via Simultaneous Ligation))
 
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== '''Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD''' (via Simultaneous Ligation) ==
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== '''Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD''' (Simultaneous Ligation) ==
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== '''Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD''' (via Sequential Ligation) ==
 
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'''''Rhodobacter sphaeroides'''''
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        '''R-bchH:''' 3582 base pairs
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        '''R-bchI''': 1005 base pairs
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        '''R-bchD''': 1695 base pairs
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'''''Cyanobacteria - Synechocystis'''''
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[[Berkeley_LBL/MimiSequence2|Sequences and Properties of Oligonucleotides for R-bch Genes]]
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[[Berkeley_LBL/Mimi_RbchHID|'''Construction of ''pET3A-(R)-bchHID''''']]
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- Not Successful
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[[Berkeley_LBL/MimiSequence3|Sequences and Properties of Oligonucleotides for R-bch Genes(NEW)]]
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[[Berkeley_LBL/Mimi_RbchHID2|'''Construction of ''pET3A-(R)-bchHID''' - 2nd Attempt'']]
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- Not Successful
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== '''Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD''' (Sequential Ligation) ==
 +
 
 +
 
 +
'''''Cyanobacteria Synechocystis'''''
         '''S-chlH:''' 3996 base pairs
         '''S-chlH:''' 3996 base pairs
         '''S-chlI''': 1074 base pairs
         '''S-chlI''': 1074 base pairs
         '''S-chlD''': 2031 base pairs
         '''S-chlD''': 2031 base pairs
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[[Berkeley_LBL/MimiSequence|Sequences and Properties of Oligonucleotides]]
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[[Berkeley_LBL/MimiSequence|Sequences and Properties of Oligonucleotides for S-chl Genes]]
[[Berkeley_LBL/Mimi-SchlH|'''Construction of ''pET3A-(S)-chlH''''']]
[[Berkeley_LBL/Mimi-SchlH|'''Construction of ''pET3A-(S)-chlH''''']]

Latest revision as of 20:29, 26 October 2007

Home Project Description Methods Notebook Results and Discussion Resources

Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD (Simultaneous Ligation)

Rhodobacter sphaeroides

       R-bchH: 3582 base pairs
       R-bchI: 1005 base pairs
       R-bchD: 1695 base pairs

Sequences and Properties of Oligonucleotides for R-bch Genes

Construction of pET3A-(R)-bchHID - Not Successful

Sequences and Properties of Oligonucleotides for R-bch Genes(NEW)

Construction of pET3A-(R)-bchHID - 2nd Attempt - Not Successful


Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD (Sequential Ligation)

Cyanobacteria Synechocystis

       S-chlH: 3996 base pairs
       S-chlI: 1074 base pairs
       S-chlD: 2031 base pairs

Sequences and Properties of Oligonucleotides for S-chl Genes

Construction of pET3A-(S)-chlH

Construction of pET3A-(S)-chlHI

Construction of pET3A-(S)-chlHID