Berkeley LBL/MimiNotebook

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Home Project Description Methods Notebook Results and Discussion Resources

Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD (via Simultaneous Ligation)

Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD (via Sequential Ligation)

Cyanobacteria - Synechocystis

       S-chlH: 3996 base pairs
       S-chlI: 1074 base pairs
       S-chlD: 2031 base pairs

Sequences and Properties of Oligonucleotides

Construction of pET3A-(S)-chlH

Construction of pET3A-(S)-chlHI

Construction of pET3A-(S)-chlHID