Boston University/Electroporation Results
From 2007.igem.org
Introduction to Electroporation and Testing
- During the weekend of 7/14/07, the BU iGEM team ran a test of Electroporation of plasmids pBAD18s, pBAD30, and pjQ200 into wildtype S. oneidensis. Plasmids pBAD18s and pBAD30 were recommended to us by Professor Gardner as possible vectors for mutated Shewy genes. pjQ200 was the plasmid our team had previously selected as a prime candidate for serving as a vector. In order to check (and in the case of pjQ200, double-check) that these plasmids are capable of direct transformation into Shewy, tests of Zymo transformation and electroporation were conducted. For both types of transformation, E. coli was used as a control to verify that our transformation procedures were conducted properly. The results are posted below in a series of images and a short analysis. Lastly, gentamycin plates were used to select for pjq200 transformants, while ampicillin plates were used to select for pBAD18s and pBAD30 transformants.