Boston University/Plasmid Customization

From 2007.igem.org

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Revision as of 14:46, 24 July 2007

Overview

Plasmid pJQ200

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ










BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg

Verifying the presence of the lacZ promoter in the plasmid pJQ200

Double digestion of pJQ200 with EcoRI and HindIII










BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg

BU labeled lacz PCR 7-24-2007.jpg

Retrieved from "http://2007.igem.org/wiki/index.php/Boston_University/Plasmid_Customization"