Calgary/testing
From 2007.igem.org
(Difference between revisions)
Revision as of 05:55, 22 October 2007
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| Choosing Our Project | Designing Our Project | Testing Parts and Primers | Constructing our Project: The Wetlab | Constructing our Project: Printer and Software | Protocols | Final Result of E.co Lisa |
I) Colony PCR test...
- Design universal primers (valid for any biobrick out of any plasmid)
- Develop massive amounts
- Gel to test
II) Test Plasmids --> always grow up 5ml of overnight
- Plasmid Prep --> isolating plasmid out of the cell
- Sigma Plasmid Prep protocol
