Calgary/testing

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< Calgary
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Choosing Our Project Designing Our Project Testing Parts and Primers Constructing our Project: The Wetlab Constructing our Project: Printer and Software Protocols Final Result of E.co Lisa

I) Colony PCR test...

  • Design universal primers (valid for any biobrick out of any plasmid)
  • Develop massive amounts
  • Gel to test

II) Test Plasmids --> always grow up 5ml of overnight

  • Plasmid Prep --> isolating plasmid out of the cell
  • Sigma Plasmid Prep protocol

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