Edinburgh/DivisionPopper

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Revision as of 14:32, 16 September 2007

MENU : Introduction | Background | Applications | Design | Realization | Modelling | Status | Conclusions

THIS PROJECT IS BEING EDITED IN THESE DAYS, YOU MAY NOTICE NOT COMPLETED PAGES OR BROKEN LINKS.

The Division PoPper is a signal generator device that produces an output of PoPS as a function of bacterial cell division. Downstream of the device may be a counter device, quantifiable protein production or some other function of choice. The system may for example perform pre-determined actions such as programmed cell death after a set number of cell divisions or being used to calculate the division frequency. The device relies on dif recombinase sites to flip a DNA segment at each cell division. We hope to further analyse cell division and recombinase mechanisms since bacterial cell division is still relatively poorly understood. We have constructed and are ligating the bricks required for the first proof of concept experiments.

The structure of the wiki for this project is composed of:

• This Introduction page with a brief overview.
• The Applications page with some possible uses of the Division PoPper.
• The References page that explain what is the scientific background of our project and lists papers validating our work.
• The Design page that explains what are the logic and functional elements in the construct.
• The Realization page that explains what are the biological elements we use to implement the construct (decoupled from design as the Synthetic Biology approach suggest).
• The Modelling page containing the computational and mathematical investigation of the system behaviour.
• The Status page that reports what we have achieved so far.
• The Conclusions page that summarize which are our final consideration on the project.

A menu with the same links is present at the top of each page for facilitating navigation.