NYMU Taipei/Costruct

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(Difference between revisions)
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 +
<h2>Assembly Ordering and Progress</h2>
 +
<h3>system 1</h3>
 +
<table style="color: green; background-color: rgb(255,255,204)" border="1">
 +
    <tbody>
 +
        <tr style="background-color: rgb(255,255,255)" align="center">
 +
            <td colspan="14"><font color="#000000">System 1: glucose-sensing insulin generator (5 task left: 2 PCR and 3 biobrick standard assembly)<br />
 +
            </font></td>
 +
        </tr>
 +
        <tr>
 +
        </tr>
 +
        <tr align="center">
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">pOmpC</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">RBS + TAT<br />
 +
            </font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">INSA</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">RBS + TAT<br />
 +
            </font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">INSB</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">RBS</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">CinR</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">RBS</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">HSL</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">D-term</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">duration</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">members</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">status</font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="2"><font color="#000000">RBS + CinR</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="2"><font color="#000000">RBS + HSL</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">8/30-8/31</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">翔</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">1st assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_1">9/01, 07</a><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="4"><font color="#000000">CinR + HSL (RBS+)</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">9/1-9/4</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">宥,君,威</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">2nd assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_2">9/02, 07</a><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="5"><font color="#000000">CinR+HSL(RBS+) + D-term</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;9/16-9/27</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">宥,君,</font><font color="#000000">翔,pett</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">3rd assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_27#CinR.2BHSL.2BD-term_check">9/27, 07</a><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="2"><font color="#000000">RBS + TATA+ INSA (PCR)</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="2"><font color="#000000">RBS + TATB+ INSB (PCR)</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)">&nbsp;<font color="#000000">9/28-9/30</font></td>
 +
            <td style="background-color: rgb(255,255,255)">
 +
            <p><font color="#000000">銘, pett<br />
 +
            </font></p>
 +
            </td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">basic part (PCR)</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_30">9/30, 07</a><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td bgcolor="#ffb6c1" colspan="3"><font color="#000000">pOmpC + TATA_INSA (failed)</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="7"><font color="#000000">TATB_INSB + CinR+HSL+D-term (wrong assembly)<br />
 +
            </font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">-10/5</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">pett</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">4th assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_5">10/5, 07</a>; 10/7, 07; 10/8, 07<br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td bgcolor="#ffb6c1" colspan="3"><font color="#000000">pOmpC + TATA_INSA</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">-10/11</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">pett</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">4th assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td bgcolor="#ffb6c1" colspan="5"><font color="#000000">pOmpC + TATA_INS_A + TATB_INS_B<br />
 +
            </font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">4th assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td bgcolor="#ffb6c1" colspan="10"><font color="#000000">pOmpC + TATA_INS_A + TATB_INS_B + CinR+HSL+D-term</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">Final<br />
 +
            </font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><br />
 +
            </font></td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>system 2</h3>
 +
<table style="color: green; background-color: rgb(255,255,204)" border="1">
 +
    <tbody>
 +
        <tr style="background-color: rgb(255,255,255)" align="center">
 +
            <td colspan="9"><font color="#000000">System 2: insulin-sensing insulin destoryer (5 tasks left: 2 PCR and 3 biobrick standard assembly)<br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">pCinRHSL</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">OmpRBS</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">RBS + TATD<br />
 +
            </font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">IDE</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">D-term</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">duration</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">members</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">status</font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td bgcolor="#ffb6c1"><font color="#000000">OmpRBS (PCR)</font></td>
 +
            <td bgcolor="#ffb6c1" colspan="2"><font color="#000000">RBS + TATD+ IDE (PCR)<br />
 +
            </font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">-102</font></td>
 +
            <td style="background-color: rgb(255,255,255)">
 +
            <p><font color="#000000">銘</font>, <font color="#000000">茹, pett<br />
 +
            </font></p>
 +
            </td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">basic part (PCR)</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_2">10/2, 07</a><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td bgcolor="#ffb6c1" colspan="2"><font color="#000000">pCinRHSL + OmpRBS</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">-10/5</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">pett</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">1st assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_5">10/5, 07</a><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td bgcolor="#ffb6c1" colspan="4"><font color="#000000">pCinRHSL + OmpRBS + TATD_IDE (RBS+)</font></td>
 +
            <td><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">-10/11</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;pett</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">2nd assembly</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><br />
 +
            </font></td>
 +
        </tr>
 +
        <tr align="center">
 +
            <td bgcolor="#ffb6c1" colspan="5"><font color="#000000">pCinRHSL+OmpRBS+TATD_IDE + D-term</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">&nbsp;</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000">Final</font></td>
 +
            <td style="background-color: rgb(255,255,255)"><font color="#000000"><br />
 +
            </font></td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<p>&nbsp;</p>
 +
<hr />
 +
<h2>Promoter assay construct: pOmpR+(RBS+EYFP)</h2>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:POmpR+EYFP.jpeg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>our first contruct for promoter activity assay under glucose stimulus</li>
 +
                <li>Insert size: 767bp</li>
 +
                <li>Total vector size: 2813bp</li>
 +
                <li>V-I: 2038bp</li>
 +
                <li>May be checked with: BtgZI</li>
 +
                <li>8/27, 2007</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<hr />
 +
<h2>System 1: pOmpC + (TATA<font color="#ff0000">+</font>INSA) + (TATB<font color="#ff0000">+</font>INSB) + (RBS<font color="#ff0000">+</font>CinR) + (RBS<font color="#ff0000">+</font>HSL) + D-term</h2>
 +
<h3>Phase 1 of system 1: RBS <font color="#ff0000">+</font> CinR</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:RBS+cinR.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>Insert size: 800bp</li>
 +
                <li>Total vector size: 2861bp</li>
 +
                <li>V-I: 2053bp</li>
 +
                <li>May be checked with: BsaBI</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>Phase 1 of system 1: RBS <font color="#ff0000">+</font> HSL</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:RBS+HSL.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>Insert size: 740bp</li>
 +
                <li>Total vector size: 2801bp</li>
 +
                <li>V-I: 2053bp</li>
 +
                <li>May be checked with: AgeI</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>Phase 1 of system 1: TATA <font color="#ff0000">+</font> INSA = TATA_INSA</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:H_INSA.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>Insert size: 106bp</li>
 +
                <li>PCR product size: 132bp</li>
 +
                <li>May be checked with: RsaI</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>Phase 1 of system 1: TATB + INSB = TATB_INSB</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:H_INSB.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>Insert size: 133bp</li>
 +
                <li>PCR product size: 159bp</li>
 +
                <li>May be checked with AluI</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>Phase 2 of system 1: pOmpC + TATA_INSA</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:POmpC+H_INSA.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>Insert size: 230bp</li>
 +
                <li>Total vector size: 2291bp</li>
 +
                <li>V-I: 2053bp</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>Phase 2 of system 1: (RBS+CinR) <font color="#ff0000">+</font> (RBS+HSL) = CinR+HSL</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:RBS+cinR+RBS+HSL.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>Insert size: 1530bp</li>
 +
                <li>Total vector size: 3591bp</li>
 +
                <li>V-I: 2053bp</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>Phase 3 of system 1: pOmpC + TATA_INSA <font color="#ff0000">+</font> TATB_INSB</h3>
 +
<h3>Phase 3 of system 1: CinR+HSL <font color="#ff0000">+</font> D-term = CinR+HSL+D-term</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:RBS+cinR+RBS+HSL+D-term.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>&nbsp;</li>
 +
                <li>item 2</li>
 +
                <li>item 3</li>
 +
                <li>item 4</li>
 +
                <li>item 5</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
<h3>Phase 3 of system 1: pOmpC + TATA_INSA + TATB_INSB <font color="#ff0000">+ </font>CinR+HSL+D-term</h3>
 +
<hr />
 +
<h2>System 2: pCinRHSL+ OmpRBS + (TATD+IDE) + D-term</h2>
 +
<h3>Phase 1 of system 2: TATD <font color="#ff0000">+</font> IDE = TATD_IDE</h3>
 +
<h3>Phase 1 of system 2: pCinRHSL <font color="#ff0000">+</font> OmpRBS</h3>
 +
<h3>Phase 2 of system 2: pCinRHSL + OmpRBS <font color="#ff0000">+</font> TATD_IDE</h3>
 +
<h3>Phase 3 of system 2: pCinRHSL + OmpRBS +&nbsp; TATD_IDE <font color="#ff0000">+</font> D-term</h3>
 +
<hr />
 +
<h2>Basic Constructs</h2>
 +
<h3>pOmpC in pSB1A2</h3>
 +
<p>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:POmpC+plasmid.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>BsmFI may be used to confirm the presence of pOmpC.</li>
 +
                <li>item 2</li>
 +
                <li>item 3</li>
 +
                <li>item 4</li>
 +
                <li>item 5</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
</p>
 +
<h3>pCinRHSL in pSB1A2</h3>
 +
<p>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:PCinRHSL(plasmid).jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>item 1</li>
 +
                <li>item 2</li>
 +
                <li>item 3</li>
 +
                <li>item 4</li>
 +
                <li>item 5</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
</p>
 +
<h3>RBS in pSB1A2</h3>
 +
<p>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:RBS+plasmid.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>BseR1 can be applied to check the insertion of RBS</li>
 +
                <li>item 2</li>
 +
                <li>item 3</li>
 +
                <li>item 4</li>
 +
                <li>item 5</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
</p>
 +
<h3>D-term in pSB1AK3</h3>
 +
<table>
 +
    <tbody>
 +
        <tr>
 +
            <td>[[Image:D-term+pSB1AK3.jpg|300px]]</td>
 +
            <td>
 +
            <ul>
 +
                <li>&nbsp;</li>
 +
                <li>&nbsp;</li>
 +
                <li>&nbsp;</li>
 +
                <li>&nbsp;</li>
 +
                <li>item 5</li>
 +
            </ul>
 +
            </td>
 +
        </tr>
 +
    </tbody>
 +
</table>
[https://2007.igem.org/NYMU_Taipei/Welcome Back]
[https://2007.igem.org/NYMU_Taipei/Welcome Back]

Revision as of 12:56, 18 October 2007

Contents

Assembly Ordering and Progress

system 1

<tbody> </tbody>
System 1: glucose-sensing insulin generator (5 task left: 2 PCR and 3 biobrick standard assembly)
pOmpC RBS + TAT
 INSA RBS + TAT
 INSB RBS CinR RBS HSL D-term duration members assembly status
          RBS + CinR RBS + HSL   8/30-8/31 翔 1st assembly <a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_1">9/01, 07</a>
          CinR + HSL (RBS+)   9/1-9/4 宥,君,威 2nd assembly <a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_2">9/02, 07</a>
          CinR+HSL(RBS+) + D-term  9/16-9/27 宥,君,翔,pett 3rd assembly <a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_27#CinR.2BHSL.2BD-term_check">9/27, 07</a>
  RBS + TATA+ INSA (PCR) RBS + TATB+ INSB (PCR)            9/28-9/30

銘, pett

 basic part (PCR) <a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_30">9/30, 07</a>
pOmpC + TATA_INSA (failed) TATB_INSB + CinR+HSL+D-term (wrong assembly)
 -10/5 pett 4th assembly <a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_5">10/5, 07</a>; 10/7, 07; 10/8, 07
pOmpC + TATA_INSA               -10/11 pett 4th assembly
pOmpC + TATA_INS_A + TATB_INS_B
               4th assembly
pOmpC + TATA_INS_A + TATB_INS_B + CinR+HSL+D-term     Final

system 2

<tbody> </tbody>
System 2: insulin-sensing insulin destoryer (5 tasks left: 2 PCR and 3 biobrick standard assembly)
pCinRHSL OmpRBS RBS + TATD
 IDE D-term duration members assembly status
  OmpRBS (PCR) RBS + TATD+ IDE (PCR)
   -102

銘, 茹, pett

 basic part (PCR) <a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_2">10/2, 07</a>
pCinRHSL + OmpRBS       -10/5 pett 1st assembly <a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_5">10/5, 07</a>
pCinRHSL + OmpRBS + TATD_IDE (RBS+)   -10/11  pett 2nd assembly
pCinRHSL+OmpRBS+TATD_IDE + D-term     Final

 

Promoter assay construct: pOmpR+(RBS+EYFP)

<tbody> </tbody>
300px
  • our first contruct for promoter activity assay under glucose stimulus
  • Insert size: 767bp
  • Total vector size: 2813bp
  • V-I: 2038bp
  • May be checked with: BtgZI
  • 8/27, 2007

System 1: pOmpC + (TATA+INSA) + (TATB+INSB) + (RBS+CinR) + (RBS+HSL) + D-term

Phase 1 of system 1: RBS + CinR

<tbody> </tbody>
300px
  • Insert size: 800bp
  • Total vector size: 2861bp
  • V-I: 2053bp
  • May be checked with: BsaBI

Phase 1 of system 1: RBS + HSL

<tbody> </tbody>
300px
  • Insert size: 740bp
  • Total vector size: 2801bp
  • V-I: 2053bp
  • May be checked with: AgeI

Phase 1 of system 1: TATA + INSA = TATA_INSA

<tbody> </tbody>
300px
  • Insert size: 106bp
  • PCR product size: 132bp
  • May be checked with: RsaI

Phase 1 of system 1: TATB + INSB = TATB_INSB

<tbody> </tbody>
300px
  • Insert size: 133bp
  • PCR product size: 159bp
  • May be checked with AluI

Phase 2 of system 1: pOmpC + TATA_INSA

<tbody> </tbody>
300px
  • Insert size: 230bp
  • Total vector size: 2291bp
  • V-I: 2053bp

Phase 2 of system 1: (RBS+CinR) + (RBS+HSL) = CinR+HSL

<tbody> </tbody>
300px
  • Insert size: 1530bp
  • Total vector size: 3591bp
  • V-I: 2053bp

Phase 3 of system 1: pOmpC + TATA_INSA + TATB_INSB

Phase 3 of system 1: CinR+HSL + D-term = CinR+HSL+D-term

<tbody> </tbody>
300px
  •  
  • item 2
  • item 3
  • item 4
  • item 5

Phase 3 of system 1: pOmpC + TATA_INSA + TATB_INSB + CinR+HSL+D-term

System 2: pCinRHSL+ OmpRBS + (TATD+IDE) + D-term

Phase 1 of system 2: TATD + IDE = TATD_IDE

Phase 1 of system 2: pCinRHSL + OmpRBS

Phase 2 of system 2: pCinRHSL + OmpRBS + TATD_IDE

Phase 3 of system 2: pCinRHSL + OmpRBS +  TATD_IDE + D-term

Basic Constructs

pOmpC in pSB1A2

<tbody> </tbody>
300px
  • BsmFI may be used to confirm the presence of pOmpC.
  • item 2
  • item 3
  • item 4
  • item 5

pCinRHSL in pSB1A2

<tbody> </tbody>
300px
  • item 1
  • item 2
  • item 3
  • item 4
  • item 5

RBS in pSB1A2

<tbody> </tbody>
300px
  • BseR1 can be applied to check the insertion of RBS
  • item 2
  • item 3
  • item 4
  • item 5

D-term in pSB1AK3

<tbody> </tbody>
300px
  •  
  •  
  •  
  •  
  • item 5

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