NYMU Taipei/Lab Notes/2007 10 16

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check for pOmpC + TATA_INSA by PCR

  • lane 1: pOmpC + TATA_INSA (clone)
  • lane 2: ligation mixture
  • there is a band (around 550 bp) larger than 430 but less than 613

digestion for (pCinRHSL+OmpRBS)

  • SpeI digestion O/N (overnight)
  • Klenow fill the sticky end into blunt end (25 C, 20 min)
  • PstI digestion
  • DNA smeared (>_<)