NYMU Taipei/Lab Notes/2007 12 12

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* pOmpC + EYFP culture
* pOmpC + EYFP culture
-
** 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin
+
** 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin (after 20hr, OD600 is 0.004)
 +
* pLacIQ + OmpRBS + GFP
 +
** re-culture pLacIQ in liquid medium (Kan+)
 +
** re-culture GFP in liquid medium ()
 +
** re-culture EYFP in liquid medium ()

Latest revision as of 04:22, 13 December 2007

  • pOmpC + EYFP culture
    • 5mL liquid culture = 5 c.c. LB + 5.0 uL stock ampicillin (after 20hr, OD600 is 0.004)
  • pLacIQ + OmpRBS + GFP
    • re-culture pLacIQ in liquid medium (Kan+)
    • re-culture GFP in liquid medium ()
    • re-culture EYFP in liquid medium ()