NYMU Taipei/Lab Notes/2007 8 6&7
From 2007.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
* mouse insulin gene cloning (delay to 8/7) | * mouse insulin gene cloning (delay to 8/7) | ||
** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus] | ** primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus] | ||
| - | *** primer for A chain | + | *** primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN) |
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font> | **** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font> | ||
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font> | **** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font> | ||
Revision as of 14:09, 6 August 2007
Lab schedule
- mouse insulin gene cloning (delay to 8/7)
- primer design for insulin II of Mus musculus
- primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN)
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
- primer for B chain
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
- primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN)
- primer design for insulin II of Mus musculus
- purchase of BCRC human insulin clone (ordered not paid)
- antibiotics LB plate preparation
- ampicillin
- kanamycin
