NYMU Taipei/Lab Notes/2007 8 6&7

From 2007.igem.org

Lab schedule

  • mouse insulin gene cloning (delay to 8/7)
    • primer design for insulin II of Mus musculus
      • primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN)
        • left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC
        • right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
      • primer for B chain
        • left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
        • right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
  • purchase of BCRC human insulin clone (ordered not paid)
  • antibiotics LB plate preparation
    • ampicillin
    • kanamycin