NYMU Taipei/Lab Notes/2007 8 9
From 2007.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
***[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription | ***[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription | ||
| - | *Transformation to competent cell | + | *Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells) |
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2 | **Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2 | ||
**Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again) | **Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again) | ||
Revision as of 13:05, 9 August 2007
- After first transformation
- Pick single colony
- BBa_R0082 OmpR
- BBa_I14032 Constitutive Promoter, High Transcription
- Pick single colony
- Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
- Part:BBa_C0082, Receptor, tar-envZ at 7O, Plate 2
- Part:BBa_I14032, Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)
