NYMU Taipei/Lab Notes/2007 8 9

From 2007.igem.org

< NYMU Taipei/Lab Notes(Difference between revisions)
 
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* After first transformation
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* Transformation results
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** pick single colony ( [http://partsregistry.org/Part:BBa_R0082 BBa_R0082 OmpR] , [http://partsregistry.org/Part:BBa_R0084 BBa_I14032 High Transcription promoter])
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**[http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : <font color="red">Only 1 colony</font>
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**[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : <font color="red">8 colonies</font>
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*Transformation to competent cell
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*Pick single colony and incubate at LB plate
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*Transformation to competent cell (Add <font color="red">2uL</font> vector to 50uL ECOS competent cells)
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
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**Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)
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**Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 <font color="red">(Transformation again)</font>

Latest revision as of 13:13, 9 August 2007

  • Transformation results
    • [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : Only 1 colony
    • [http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : 8 colonies
  • Pick single colony and incubate at LB plate
  • Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
    • Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
    • Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)