NYMU Taipei/Lab Notes/2007 9 1

From 2007.igem.org

(Difference between revisions)
Line 5: Line 5:
**RBS+HSL 2: 8ug/ul
**RBS+HSL 2: 8ug/ul
**RBS+HSL 3: 6ug/ul
**RBS+HSL 3: 6ug/ul
-
*Enzyme digest RBS+cinR 1, RBS+HSL 2, RBS+HSL 3
+
*Enzyme digest RBS+cinR 1
 +
**RBS+cinR 1: 14ul
 +
**SpeI: 1ul
 +
**PstI 1ul
 +
**10x buffer 2: 2ul
 +
**10x BSA: 2ul
 +
**37°C water bath, 2h
 +
**Heat inactivation: 65°C, 20min
 +
*Enzyme digest RBS+HSL 2
 +
**RBS+HSL 2: 14ul
 +
**XbaI: 1ul
 +
**PstI: 1ul
 +
**10x buffer 3: 2ul
 +
**10x BSA: 2ul
 +
**37°C water bath, 2h
 +
**Heat inactivation: 65°C, 20min
 +
*Enzyme digest RBS+HSL 3
 +
**RBS+HSL 3: 14ul
 +
**XbaI: 1ul
 +
**PstI: 1ul
 +
**10x buffer 3: 2ul
 +
**10x BSA: 2ul
 +
**37°C water bath, 2h
 +
**Heat inactivation: 65°C, 20min
 +
*Check RBS+cinR 1 with BsaBI digestion

Revision as of 08:23, 1 September 2007

  • Plasmid extraction: RBS+cinR 1, RBS+HSL 1, RBS+HSL 2, RBS+HSL 3 in 50ul EB
  • The concentration was measured with spectrophotometer at 260nm
    • RBS+cinR 1: 8ug/ul
    • RBS+HSL 1: 3ug/ul
    • RBS+HSL 2: 8ug/ul
    • RBS+HSL 3: 6ug/ul
  • Enzyme digest RBS+cinR 1
    • RBS+cinR 1: 14ul
    • SpeI: 1ul
    • PstI 1ul
    • 10x buffer 2: 2ul
    • 10x BSA: 2ul
    • 37°C water bath, 2h
    • Heat inactivation: 65°C, 20min
  • Enzyme digest RBS+HSL 2
    • RBS+HSL 2: 14ul
    • XbaI: 1ul
    • PstI: 1ul
    • 10x buffer 3: 2ul
    • 10x BSA: 2ul
    • 37°C water bath, 2h
    • Heat inactivation: 65°C, 20min
  • Enzyme digest RBS+HSL 3
    • RBS+HSL 3: 14ul
    • XbaI: 1ul
    • PstI: 1ul
    • 10x buffer 3: 2ul
    • 10x BSA: 2ul
    • 37°C water bath, 2h
    • Heat inactivation: 65°C, 20min
  • Check RBS+cinR 1 with BsaBI digestion
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