NYMU Taipei/Lab Notes/2007 9 11
From 2007.igem.org
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** two different PCR (INS_A and INS_B) have been done by 海威 (9/5, 2007) and 惇茹 (9/8, 2007) | ** two different PCR (INS_A and INS_B) have been done by 海威 (9/5, 2007) and 惇茹 (9/8, 2007) | ||
** we had failed three times for construction of pOmpC + INS_A using 惇茹's INS_A and following three different ligation protocol | ** we had failed three times for construction of pOmpC + INS_A using 惇茹's INS_A and following three different ligation protocol | ||
| - | *** insert (1 uL) vs. vector (1 uL) | + | *** insert (1 uL) vs. vector (1 uL) at 09/09, 2007 |
| - | *** insert (2 uL) vs. vector (1 uL) | + | *** insert (2 uL) vs. vector (1 uL) at 09/10, 2007 |
| - | *** insert (3 uL) vs. vector (1 uL) | + | *** insert (3 uL) vs. vector (1 uL) at 09/10, 2007 |
| + | *** a trivial failure (absent of vector part) at 09/08, 2007 | ||
Revision as of 14:34, 11 September 2007
- Fresh back
- two different PCR (INS_A and INS_B) have been done by 海威 (9/5, 2007) and 惇茹 (9/8, 2007)
- we had failed three times for construction of pOmpC + INS_A using 惇茹's INS_A and following three different ligation protocol
- insert (1 uL) vs. vector (1 uL) at 09/09, 2007
- insert (2 uL) vs. vector (1 uL) at 09/10, 2007
- insert (3 uL) vs. vector (1 uL) at 09/10, 2007
- a trivial failure (absent of vector part) at 09/08, 2007
