NYMU Taipei/Lab Notes/2007 9 29

From 2007.igem.org

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{| align=center | border=1 | style="text-align:center"
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|Template
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|1uL
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|-
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|10X buffer
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|5uL
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|-
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|2.5mM dNTP
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|2uL
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|-
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|Primer_F
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|1.5uL
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|-
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|Primer_R
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|1.5uL
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|-
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|pfu polymerase
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|0.5uL
 +
|-
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|ddH2O
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|38.5uL
 +
|-
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|Total
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|50uL
 +
|-

Revision as of 12:40, 29 September 2007

PCR

size Primer Template
1 TAT_A 140bp TAT_F+TAT_A_R E.coli K12 genome DNA
2 INS_A 111bp INS_A_F+INS_A_R MGC clone (H_INS 1)
3 TAT_B 137bp TAT_F+TAT_B_R E.coli K12 genome DNA
4 INS_B 135bp INS_B_F+INS_B_R MGC clone (H_INS 1)
5 TAT_D 131bp TAT_F+TAT_D_R E.coli K12 genome DNA
6 OmpRBS 100bp OmpRBS_F+OmpRBS_R pOmpC
Template 1uL
10X buffer 5uL
2.5mM dNTP 2uL
Primer_F 1.5uL
Primer_R 1.5uL
pfu polymerase 0.5uL
ddH2O 38.5uL
Total 50uL
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