NYMU Taipei/Lab Notes/2007 9 30
From 2007.igem.org
PCR for TAT_A-INS_A TAT_B-INS_B
size | Primer | Template | ||
---|---|---|---|---|
1 | TAT_A-INS_A | TAT_F+INS_A_R | TAT_A,INS_A PCR product@9/29 | |
2 | TAT_B-INS_B | TAT_F+INS_B_R | TAT_B,INS_B PCR product@9/29 |
Step | Temp (°C) | Time (mins) | Cycle |
---|---|---|---|
Initial Denaturation | 95 | 10 | |
Denatuation Annealing Extension | 95 55 72 | 1 1 1 | 35 |
Final Extension | 72 | 10 | |
Soak | 4 |
Template1 | 1uL |
Template2 | 1uL |
10X buffer | 5uL |
2.5mM dNTP | 2uL |
Primer_F | 1.5uL |
Primer_R | 1.5uL |
Pfu polymerase | 0.5uL |
ddH2O | 37.5uL |
Total | 50uL |