NYMU Taipei/Primer List

From 2007.igem.org

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</font><br /><hr></p>
</font><br /><hr></p>
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{| style="color:green;background-color:#ffffcc;" cellpadding="20" cellspacing="0" border="1"
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{| style="color:green;background-color:#ffffcc;" cellpadding="20" cellspacing="0" border="1" width="100%"
!Name
!Name
!Sequence
!Sequence
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|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">ATGAACAATAACGATCTCTTTCAGG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">ATGAACAATAACGATCTCTTTCAGG</font>
|56 bp
|56 bp
 +
|59.92
 +
|XbaI
 +
| bp
 +
|XbaI+RBS
 +
|-
 +
|-
 +
|TAT F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">ATGAACAATAACGATCTCTTTCAGG</font>
 +
|108 bp
|59.92
|59.92
|XbaI
|XbaI
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|-
|-
|H_INS_A F
|H_INS_A F
-
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
+
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|54 bp
|54 bp
|62.45
|62.45
|XbaI
|XbaI
|122 bp
|122 bp
-
|Potential dimer formation! XbaI+RBS, ATG removed.
+
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|H_INS_A TAT F
 +
|<font color="green">CGCTTGCGCCGCAGTCGCAC</font><font color="red">GGCATTGTGGAACAATGCTG</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|
|-
|-
-
|TAT signal H_INS_A R
+
|TAT H_INS_A R
|<font color="green">CAGCATTGTTCCACAATGCC</font><font color="red">CGCTTGCGCCGC</font>
|<font color="green">CAGCATTGTTCCACAATGCC</font><font color="red">CGCTTGCGCCGC</font>
|32 bp
|32 bp
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|149 bp
|149 bp
|Potential dimer formation! XbaI+RBS
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|H_INS_B TAT F
 +
|<font color="green">CGCTTGCGCCGCAGTCGCAC</font><font color="red">TTTGTGAACCAACACCTGTG</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|
 +
|-
 +
|TAT H_INS_B R
 +
|<font color="green">CACAGGTGTTGGTTCACAAA</font><font color="red">CGCTTGCGCCGC</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|
 +
|-
|-
|-
|-
|-
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|Reverse sequencing primer.
|Reverse sequencing primer.
|-
|-
-
|M_IDE F
+
|M_IDE TAT F
-
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
+
|<font color="green">CGCTTGCGCCGCAGTCGCAC</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
-
|54 bp
+
|40 bp
 +
|
|
|
-
|XbaI
 
| bp
| bp
-
|Potential dimer formation! XbaI+RBS
+
|Potential dimer formation!
 +
|-
 +
|TAT M_IDE R
 +
|<font color="green">AGCCACACGAGCCCGTTCCG</font><font color="red">CGCTTGCGCCGC</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|
 +
|-
|-
|-
|-
|-
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| bp
| bp
|Potential dimer formation!
|Potential dimer formation!
 +
|-
 +
|-
 +
|OmpRBS F
 +
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGAG</font><font color="red">TACATTTTGAAACATCTATAGCG</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|Primer dimer
 +
|-
 +
|OmpRBS R
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTA</font><font color="red">ACGAATATGATACTAAAACTTTTAAGAT</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|Primer dimer
 +
|-
 +
|TAT signal peptide
 +
|<font color="lightseagreen">ATG gacaaattcgacgct</font><font color="black">aatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctg</font><font color=purple>ccgacccctgcgtttgca</font>
 +
|90 bp
 +
|
 +
|
 +
|
 +
|TAT signal peptide for transport protein expressed in ''E.coli''
 +
|-
 +
|TAT_FP
 +
|<font color="black">AAG TCTAGA aaagaggagaaa ACTAG </font><font color="lightseagreen">ATG gacaaattcgacgc </font>
 +
|44 - 1 bp
 +
|55.26
 +
|XbaI
 +
|90 bp
 +
|TAT signal peptide forward primer
 +
|-
 +
|TAT_RP
 +
|<font color="orange">GCATTGTTCCACAATGCC </font><font color="purple">tgcaaacgcaggggt</font>
 +
|36 -3 bp
 +
|58.05
 +
|potential dimer (overlap 10)
 +
|90 bp
 +
|TAT signal peptide reverse primer
 +
|-
 +
|INS_A
 +
|<font color="orange">GGCATTGTGGAACAATGC </font><font color="black">TGTACCAGCATCTGCTCCCTCTACCAGCTGGAG </font><font color=greenyellow>AACTACTGCAAC <b>TAG</b></font>
 +
|63 + 3 bp
 +
|
 +
|
 +
|
 +
|human insulin A chain
 +
|-
 +
|TAT_INS_A_FP
 +
|<font color="purple">ccgacccctgcgtttgca </font><font color="orange">GGCATTGTGGAACAATGC </font>
 +
|36 bp
 +
|58.40
 +
|potential dimer (overlap 17)
 +
|63 bp
 +
|INS_A forward primer with TAT
 +
|-
 +
|TAT_INS_A_RP
 +
|<font color="black"><b>ctgcag</b> cggccgct <b>actagt</b> a <b>TTA TTA</b>  </font><font color="greenyellow">CTAGTTGCAGTAGTT </font><font color=black>CTCCAG</font>
 +
|42 + 6 bp
 +
|52.05
 +
|SpeI, PstI
 +
|63 bp
 +
|INS_A reverse primer with TAT
|-
|-
|}
|}
* [http://www.mdbio.com.tw/pfu.htm pfu primer]
* [http://www.mdbio.com.tw/pfu.htm pfu primer]
* [http://partsregistry.org/Help:Contents Biobrick Help]
* [http://partsregistry.org/Help:Contents Biobrick Help]
 +
* Tm is estimated by Amplify 3

Latest revision as of 10:03, 20 September 2007

Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) RE site Target length Description
M_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation!
M_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT 55 bp 60.85 XbaI 149 bp Potential dimer formation!
M_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation!
TAT F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG 56 bp 59.92 XbaI bp XbaI+RBS
TAT F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG 108 bp 59.92 XbaI bp XbaI+RBS
H_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG 54 bp 62.45 XbaI 122 bp Potential dimer formation! XbaI+RBS
H_INS_A TAT F CGCTTGCGCCGCAGTCGCACGGCATTGTGGAACAATGCTG bp bp
TAT H_INS_A R CAGCATTGTTCCACAATGCCCGCTTGCGCCGC 32 bp 59.26 bp Potential dimer formation!
H_INS_A R' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation!
H_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG 54 bp 57.98 XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B TAT F CGCTTGCGCCGCAGTCGCACTTTGTGAACCAACACCTGTG bp bp
TAT H_INS_B R CACAGGTGTTGGTTCACAAACGCTTGCGCCGC bp bp
H_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp
RcsC F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG 54 bp 55.92 XbaI 1404 bp XbaI+RBS
RcsC R GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
VF2 tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
VR attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
M_IDE TAT F CGCTTGCGCCGCAGTCGCACCGGAACGGGCTCGTGTGGCT 40 bp bp Potential dimer formation!
TAT M_IDE R AGCCACACGAGCCCGTTCCGCGCTTGCGCCGC bp bp
M_IDE R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP3 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP3 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP7 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP7 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
OmpRBS F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACATTTTGAAACATCTATAGCG bp bp Primer dimer
OmpRBS R GTTTCTTCCTGCAGCGGCCGCTACTAGTAACGAATATGATACTAAAACTTTTAAGAT bp bp Primer dimer
TAT signal peptide ATG gacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgca 90 bp TAT signal peptide for transport protein expressed in E.coli
TAT_FP AAG TCTAGA aaagaggagaaa ACTAG ATG gacaaattcgacgc 44 - 1 bp 55.26 XbaI 90 bp TAT signal peptide forward primer
TAT_RP GCATTGTTCCACAATGCC tgcaaacgcaggggt 36 -3 bp 58.05 potential dimer (overlap 10) 90 bp TAT signal peptide reverse primer
INS_A GGCATTGTGGAACAATGC TGTACCAGCATCTGCTCCCTCTACCAGCTGGAG AACTACTGCAAC TAG 63 + 3 bp human insulin A chain
TAT_INS_A_FP ccgacccctgcgtttgca GGCATTGTGGAACAATGC 36 bp 58.40 potential dimer (overlap 17) 63 bp INS_A forward primer with TAT
TAT_INS_A_RP ctgcag cggccgct actagt a TTA TTA CTAGTTGCAGTAGTT CTCCAG 42 + 6 bp 52.05 SpeI, PstI 63 bp INS_A reverse primer with TAT
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