NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
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|H_INS_A F
|H_INS_A F
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|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
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|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|54 bp
|54 bp
|62.45
|62.45
|XbaI
|XbaI
|122 bp
|122 bp
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|Potential dimer formation! XbaI+RBS, ATG removed.
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|Potential dimer formation! XbaI+RBS
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|-
|H_INS_A TAT F
|H_INS_A TAT F
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|TAT signal H_INS_A R
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|TAT H_INS_A R
|<font color="green">CAGCATTGTTCCACAATGCC</font><font color="red">CGCTTGCGCCGC</font>
|<font color="green">CAGCATTGTTCCACAATGCC</font><font color="red">CGCTTGCGCCGC</font>
|32 bp
|32 bp
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|149 bp
|149 bp
|Potential dimer formation! XbaI+RBS
|Potential dimer formation! XbaI+RBS
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|-
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|H_INS_B TAT F
 +
|<font color="green">CGCTTGCGCCGCAGTCGCAC</font><font color="red">TTTGTGAACCAACACCTGTG</font>
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|bp
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|bp
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|TAT H_INS_B R
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|<font color="green">CACAGGTGTTGGTTCACAAA</font><font color="red">CGCTTGCGCCGC</font>
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|bp
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|bp
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|Reverse sequencing primer.
|Reverse sequencing primer.
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|M_IDE F
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|M_IDE TAT F
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|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
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|<font color="green">CGCTTGCGCCGCAGTCGCAC</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
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|54 bp
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|40 bp
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|XbaI
 
| bp
| bp
-
|Potential dimer formation! XbaI+RBS
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|Potential dimer formation!
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|-
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|TAT M_IDE R
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|<font color="green">AGCCACACGAGCCCGTTCCG</font><font color="red">CGCTTGCGCCGC</font>
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|bp
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|bp
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Revision as of 10:28, 13 September 2007

Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) RE site Target length Description
M_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation!
M_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT 55 bp 60.85 XbaI 149 bp Potential dimer formation!
M_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation!
TAT F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG 56 bp 59.92 XbaI bp XbaI+RBS
H_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG 54 bp 62.45 XbaI 122 bp Potential dimer formation! XbaI+RBS
H_INS_A TAT F CGCTTGCGCCGCAGTCGCACGGCATTGTGGAACAATGCTG bp bp
TAT H_INS_A R CAGCATTGTTCCACAATGCCCGCTTGCGCCGC 32 bp 59.26 bp Potential dimer formation!
H_INS_A R' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation!
H_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG 54 bp 57.98 XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B TAT F CGCTTGCGCCGCAGTCGCACTTTGTGAACCAACACCTGTG bp bp
TAT H_INS_B R CACAGGTGTTGGTTCACAAACGCTTGCGCCGC bp bp
H_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp
RcsC F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG 54 bp 55.92 XbaI 1404 bp XbaI+RBS
RcsC R GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2] tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR] attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
M_IDE TAT F CGCTTGCGCCGCAGTCGCACCGGAACGGGCTCGTGTGGCT 40 bp bp Potential dimer formation!
TAT M_IDE R AGCCACACGAGCCCGTTCCGCGCTTGCGCCGC bp bp
M_IDE R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP3 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP3 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP7 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP7 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
  • [http://www.mdbio.com.tw/pfu.htm pfu primer]
  • [http://partsregistry.org/Help:Contents Biobrick Help]
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